In situ Electron Microscopy of Complex Biological and Nanoscale Systems: Challenges and Opportunities
In situ Electron Microscopy of Complex Biological and Nanoscale Systems: Challenges and Opportunities
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复杂生物和纳米系统的原位电子显微镜：挑战和机遇
DOI：
10.3389/fnano.2020.606253
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Zexiang Han-;A. Porter
中科院分区：
文献类型：
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作者：
Zexiang Han-;A. Porter
用于直接可视化的原位成像对于物理和生物科学是重要的。阐明动态生物学和纳米级现象的研究努力经常需要原位和时间分辨成像。原位液池电子显微镜（LC-EM）可以克服传统电子显微镜的某些局限性，并提供了很大的希望。本文综述了原位LC-EM及其应用的现状和实际挑战，并提供了一种新的相关技术称为微流控液体细胞电子显微镜的见解。最后，我们提出了一些研究思路，采用微流控LC-EM原位成像的生物和纳米系统。
In situ imaging for direct visualization is important for physical and biological sciences. Research endeavors into elucidating dynamic biological and nanoscale phenomena frequently necessitate in situ and time-resolved imaging. In situ liquid cell electron microscopy (LC-EM) can overcome certain limitations of conventional electron microscopies and offer great promise. This review aims to examine the status-quo and practical challenges of in situ LC-EM and its applications, and to offer insights into a novel correlative technique termed microfluidic liquid cell electron microscopy. We conclude by suggesting a few research ideas adopting microfluidic LC-EM for in situ imaging of biological and nanoscale systems.