Emergence of diverse patterns driven by molecular motors in the motility assay.
Emergence of diverse patterns driven by molecular motors in the motility assay.
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运动测定中分子马达驱动的多种模式的出现。
DOI：
10.1002/cm.21808
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kim,Taeyoon
中科院分区：
文献类型：
--
作者：
Slater,Brandon;Jung,Wonyeong;Kim,Taeyoon
肌动球蛋白的收缩力源于肌动蛋白骨架中肌动蛋白和肌动蛋白马达之间的相互作用，产生机械力并驱动广泛的细胞过程，包括细胞迁移和细胞分裂。为了探测肌动蛋白和肌凝蛋白马达之间的相互作用，肌凝蛋白运动试验已被广泛采用，其中包括附着在玻璃表面的肌凝蛋白头和通过与头的相互作用在表面上滑动的F -肌动蛋白。一些实验表明，由于相邻F - actin之间的体积排斥效应，F - actin以集体方式移动。此外，计算模型显示了关键参数的变化如何导致运动分析中不同模式的形成。然而，在大多数计算模型中，通过对细丝施加恒定的推进力来减少计算成本，隐含地考虑了肌凝蛋白马达。这种简化限制了模型提供的见解的生理相关性，并可能导致人为因素。在这项研究中，我们采用了一种基于试剂的计算模型来进行运动分析，其中明确的固定马达与细丝相互作用。我们严格地解释了肌凝蛋白马达的动力学，包括行走的力-速度关系以及结合和解除结合行为。我们探讨了长丝的长度、刚性、浓度和排斥力对集体运动和图案形成的影响。研究发现，四种不同类型的结构——均匀网络、群、带和环——是滑动细丝之间碰撞的结果。我们进一步分析了这些结构的频率和形态，以及细丝的曲率、排列和旋转运动。我们的研究提供了更好的见解，以起源和性质形成的图案由滑动细丝超越了以前的显示。
Actomyosin contractility originating from interactions between F‐actin and myosin motors in the actin cytoskeleton generates mechanical forces and drives a wide range of cellular processes including cell migration and cytokinesis. To probe the interactions between F‐actin and myosin motors, the myosin motility assay has been popularly employed, which consists of myosin heads attached to a glass surface and F‐actins gliding on the surface via interactions with the heads. Several experiments have shown that F‐actins move in a collective fashion due to volume‐exclusion effects between neighboring F‐actins. Furthermore, Computational models have shown how changes in key parameters lead to diverse pattern formation in motility assay. However, in most of the computational models, myosin motors were implicitly considered by applying a constant propulsion force to filaments to reduce computational cost. This simplification limits the physiological relevance of the insights provided by the models and potentially leads to artifacts. In this study, we employed an agent‐based computational model for the motility assay with explicit immobile motors interacting with filaments. We rigorously account for the kinetics of myosin motors including the force‐velocity relationship for walking and the binding and unbinding behaviors. We probed the effects of the length, rigidity, and concentration of filaments and repulsive strength on collective movements and pattern formation. It was found that four distinct types of structures—homogeneous networks, flocks, bands, and rings—emerged as a result of collisions between gliding filaments. We further analyzed the frequency and morphology of these structures and the curvature, alignment, and rotational motions of filaments. Our study provides better insights into the origin and properties of patterns formed by gliding filaments beyond what was shown before.
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影响因子：
3.4
作者：
Freedman, Simon L.;Banerjee, Shiladitya;Dinner, Aaron R.
通讯作者：
Dinner, Aaron R.
平滑肌肌球蛋白和骨骼肌肌球蛋白在体外零负荷下均表现出低占空比。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Harris,DE;Warshaw,DM
通讯作者：
Warshaw,DM
DOI：
10.1103/physreve.97.022606
发表时间：
2016-08
期刊：
Physical review. E
影响因子：
--
作者：
K. Prathyusha;S. Henkes;R. Sknepnek
通讯作者：
K. Prathyusha;S. Henkes;R. Sknepnek
影响因子：
56.9
作者：
Huber, L.;Suzuki, R.;Bausch, A. R.
通讯作者：
Bausch, A. R.
影响因子：
3.5
作者：
Kim, Taeyoon
通讯作者：
Kim, Taeyoon