Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin.
Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin.
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发表时间：
2007
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通讯作者：
K. Fairley;R. Purdy;S. Kearns;S. Anderson;B. Meade
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作者：
K. Fairley;R. Purdy;S. Kearns;S. Anderson;B. Meade
这些研究是为了研究皮肤暴露于全氟辛酸(PFOA)对卵蛋白(OVA)在哮喘小鼠模型中的过敏反应的作用。全氟辛烷磺酸被广泛用作地毯和织物保护剂。BALB/c小鼠每只耳的背部皮肤暴露于0.01%~1.5%浓度的全氟辛酸(剂量0.25~50 mg/kg)，连续4天。在过敏研究中，小鼠还在第1天和第10天ip注射7.5微克OVA和2 mg明矾，在一些研究中，在第17天和第26天通过咽吸吸250微克OVA。在接触全氟辛烷磺酸后，观察到肝脏重量增加，胸腺和脾重量以及细胞减少。联合应用全氟辛酸和卵清蛋白的小鼠也表现出类似的免疫调节趋势。与OVA单独暴露的动物相比，当OVA和浓度从0.75%到1.5%的PFOA共同暴露时，小鼠的总IgE增加，而OVA特异性的IgE反应在0.75%的PFOA共同暴露时达到峰值(p<；or=0.05)。与单独接触OVA的动物相比，1.0%PFOA联合暴露组的OVA特异性气道高反应性增加(P<；OR=0.05)，与单独暴露的动物相比，联合暴露的动物具有以嗜酸性粒细胞和粘蛋白产生为特征的多效性细胞反应增加。在小鼠模型中，全氟辛酸在皮肤暴露后被证明是免疫毒性的，并增强了对OVA的超敏反应，这表明全氟辛酸暴露可能增强了对环境过敏原的IgE反应。
These studies were conducted to investigate the role of dermal exposure to perfluorooctanoic acid (PFOA), a known immunosuppressant, on the hypersensitivity response to ovalbumin (OVA) in a murine model of asthma. PFOA has had widespread use as a carpet and fabric protectant. BALB/c mice were exposed dermally, on the dorsal surface of each ear, to concentrations of PFOA ranging from 0.01 to 1.5% (applied dose 0.25-50 mg/kg) for 4 days. In hypersensitivity studies, mice were also ip injected with 7.5 microg OVA and 2 mg alum on days 1 and 10 and in some studies challenged with 250 microg OVA by pharyngeal aspiration on days 17 and 26. Following exposure to PFOA, an increase in liver weights and a decrease in thymus and spleen weights and cellularities were observed. Similar immunomodulatory trends were demonstrated in mice coadministered PFOA and OVA. Compared to the OVA alone-exposed animals, an increase in total IgE was demonstrated when mice were coexposed to OVA and concentrations of PFOA ranging from 0.75 to 1.5%, while the OVA-specific IgE response peaked with 0.75% PFOA coexposure (p < or = 0.05). OVA-specific airway hyperreactivity was increased in the 1.0% PFOA coexposed group (p < or = 0.05), with an increased pleiotropic cell response characterized by eosinophilia and mucin production, in animals coexposed to concentrations of PFOA up to 1.0%, as compared to the OVA alone-exposed animals. In a murine model, PFOA was demonstrated to be immunotoxic following dermal exposure, with an enhancement of the hypersensitivity response to OVA, suggesting that PFOA exposure may augment the IgE response to environmental allergens.