REGULATION OF CELLULAR PHENOTYPE AND EXPRESSION OF POLYOMAVIRUS MIDDLE T-ANTIGEN IN RAT FIBROBLASTS
REGULATION OF CELLULAR PHENOTYPE AND EXPRESSION OF POLYOMAVIRUS MIDDLE T-ANTIGEN IN RAT FIBROBLASTS
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DOI：
10.1128/mcb.5.9.2476
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
BENJAMIN, TL
中科院分区：
文献类型：
--
作者：
RAPTIS, L;LAMFROM, H;BENJAMIN, TL
在地塞米松可调控的小鼠乳腺肿瘤病毒启动子的控制下，在大鼠F-111细胞中表达多瘤中T抗原（mT）。分级表型反应水平的mT诱导的激素，形态转化，焦点形成和锚定依赖性生长需要增加mT的表达水平。不同克隆形成肿瘤的能力反映了它们诱导mT相关激酶的最大水平和它们在软琼脂中生长的能力。转化参数和致瘤性的表达与免疫复合物中pp 60 c-src磷酸化的mT水平相关，而与代谢标记确定的mT总量无关。有人建议，细胞因子调节mT活性，形成一个激酶活性部分的mT分子控制的转化状态。
Polyoma middle T antigen (mT) was expressed in rat F-111 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promoter. Graded phenotypic responses to levels of mT induction by the hormone were seen, with morphological transformation, focus formation and anchorage-dependent growth requiring increasing levels of mT expression. The ability of different clones to form tumors reflected their maximum level of induction of mT-associated kinase and their ability to grow in soft agar. Expression of transformation parameters and tumorigenicity correlates with the level of mT phosphorylated by pp60c-src in immune complexes and not with the total amount of mT determined by metabolic labeling. It is suggested that cellular factors regulate mT activity by forming a kinase-active fraction of mT molecules that controls the transformed state.