Differential role for IL-7 in inducing lung Kruppel-like factor (Kruppel-like factor 2) expression by naive versus activated T cells
Differential role for IL-7 in inducing lung Kruppel-like factor (Kruppel-like factor 2) expression by naive versus activated T cells
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DOI：
10.1093/intimm/dxg133
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发表时间：
2003-11-01
影响因子：
4.4
通讯作者：
Jameson, SC
中科院分区：
文献类型：
--
作者：
Endrizzi, BT;Jameson, SC
Kruppel样因子2 [KLF 2，也称为肺Kruppel样因子（LKLF）]是一种转录因子，显示对于维持幼稚T细胞是必需的。KLF 2在幼稚细胞和记忆细胞中都有表达，并被认为可以促进这些细胞群的静止。在T细胞刺激过程中，KLF 2蛋白和mRNA都下调，KLF 2的丢失似乎对T细胞的完全激活至关重要。然而，目前尚不清楚KLF 2表达是如何在初始T细胞中维持的。最近有人提出，已知促进抗原刺激的T细胞中的KLF 2再表达的IL-7也可以诱导幼稚T细胞中的KLF 2表达。在这里，我们通过比较IL-7对幼稚和活化T细胞中KLF 2表达的影响来解决这个问题。使用bcl-72转基因T细胞使我们能够将IL-7在维持幼稚T细胞存活中的作用与其在维持KLF 2表达中的作用分开。我们的数据表明，IL-7信号是不需要的幼稚T细胞中的KLF 2的维护，这表明这种细胞因子有不同的影响KLF 2表达幼稚与活化的T细胞。
Kruppel-like Factor 2 [KLF2, also called lung Kruppel-like factor (LKLF)] is a transcription factor shown to be necessary for the maintenance of naive T cells. KLF2 is expressed in both naive and memory cells, and is proposed to promote quiescence in these populations. During T cell stimulation, both KLF2 protein and mRNA are down-regulated, and loss of KLF2 appears to be critical for full T cell activation. It is unclear, however, how KLF2 expression is maintained in naive T cells. Recently it was proposed that IL-7, which is known to promote KLF2 re-expression in antigen-stimulated T cells, may also induce KLF2 expression in naive T cells. Here we address this issue by comparing the impact of IL-7 on KLF2 expression in naive and activated T cells. Use of bcl-72 transgenic T cells allowed us to uncouple the requirements for IL-7 in preserving naive T cell survival from its role in maintaining KLF2 expression. Our data demonstrates that IL-7 signals are not required for KLF2 maintenance in naive T cells, suggesting that this cytokine has distinct effects on KLF2 expression in naive versus activated T cells.