Analysis of Genetic Diversity of Russian Sour Cherry Plum pox virus Isolates Provides Evidence of a New Strain
Analysis of Genetic Diversity of Russian Sour Cherry Plum pox virus Isolates Provides Evidence of a New Strain
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DOI：
10.1094/pdis-07-17-1104-re
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发表时间：
2018-03-01
期刊：
影响因子：
4.5
通讯作者：
Zakubanskiy, Alexander
中科院分区：
文献类型：
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作者：
Chirkov, Sergei;Sheveleva, Anna;Zakubanskiy, Alexander
李痘病毒（PPV）是由9个适应不同李属寄主的株系组成的复合体。在俄罗斯的酸樱桃上发现了不属于已知樱桃适应株的不寻常的PPV分离株。在此，通过在Illumina HiSeq 2500平台上测序来确定分离株达特-2和达特-4的两个完整基因组。两者均由9，792个核苷酸组成，不包括poly（A）尾，具有PPV典型的组织结构，在核苷酸和氨基酸水平上彼此之间具有99.4%和99.7%的同一性。达特-2/达特-4与已知PPV毒株的基因组RNA序列同源性为77.6%~ 83.3%，多聚蛋白序列同源性为80.0%~ 93.8%。系统发育分析将达特-2和达特-4置于一个单独的Glade中，与C和CR菌株不同。另外3个达特-2/达特-4-like菌株在当地樱桃种植使用新开发的，具体的RT-PCR检测。基于系统发育分析、序列一致性和环境分布，达特-2、达特-4和相关分离物代表了一种新的樱桃适应性PPV株系，建议将其命名为PPV CV（樱桃伏尔加）。
Plum pox virus (PPV) exists as a complex of nine strains adapted to different Prunus hosts. Unusual PPV isolates that do not belong to the known cherry-adapted strains were discovered on sour cherry in Russia. Here, two complete genomes of isolates Tat-2 and Tat-4 were determined by sequencing on the Illumina HiSeq 2500 platform. Both were composed of 9,792 nucleotides, excluding the poly(A) tail, with the organization typical of PPV and had 99.4 and 99.7% identity between each other at the nucleotide and amino acid levels. The sequence identities between Tat-2/Tat-4 and known PPV strains ranged from 77.6 to 83.3% for genomic RNA and from 80.0 to 93.8% for polyprotein. Phylogenetic analysis placed Tat-2 and Tat-4 in a separate Glade, distinct from the C and CR strains. Three more Tat-2/Tat-4-like isolates were detected in local cherry plantings using the newly developed, specific RT-PCR assay. Based on the phylogenetic analysis, sequence identities, and environmental distribution, Tat-2, Tat-4, and related isolates represent a new cherry-adapted PPV strain for which the name PPV-CV (Cherry Volga) is proposed.