Pollen generates nitric oxide and nitrite: a possible link to pollen-induced allergic responses
Pollen generates nitric oxide and nitrite: a possible link to pollen-induced allergic responses
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DOI：
10.1016/j.plaphy.2008.09.005
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发表时间：
2009-01-01
影响因子：
6.5
通讯作者：
Hancock, John T.
中科院分区：
文献类型：
--
作者：
Bright, Jo;Hiscock, Simon J.;Hancock, John T.
活性氮物种(RN)，如一氧化氮(NO)，是广泛存在于动植物体内的多种信号分子，参与多种生理和病理生理过程。亚硝酸盐是NO代谢的产物，近年来也被认为是哺乳动物细胞基本生理机制的重要介体，也是几个植物细胞信号转导过程中产生NO的底物。先前的研究表明，在植物生殖过程中，细胞内的NO是由花粉产生的，这种NO在花粉和柱头之间的相互作用中可能是重要的信号。这项研究的目的是利用广泛的植物物种来确定花粉是否释放NO和亚硝酸盐。结合电子顺磁共振(EPR)光谱分析，本研究表明，所有受检的水化花粉都释放了NO，尽管有些似乎比其他的更有活性。此外，基于臭氧的气相化学发光数据显示，亚硝酸盐也从水合花粉中释放出来。鉴于花粉与其他细胞有相互作用，例如在人类的过敏性鼻炎(花粉热)中，这表明NO可能参与了植物和动物细胞对花粉的反应。这些发现可能对未来的过敏研究具有重要意义，因为在花粉诱导的过敏反应中，花粉来源的NO和亚硝酸盐可能会影响哺乳动物细胞。(C)2008年爱思唯尔·马森公司。版权所有。
Reactive nitrogen species (RNS), such as nitric oxide (NO), are ubiquitous and diverse signalling molecules involved in a wide range of physiological and pathophysiological processes in both animals and plants. Nitrite, a metabolite of NO turnover, has also been recently characterised as an important mediator of fundamental physiological mechanisms in mammalian cells, and is a substrate for NO production in several plant cell signalling processes. A previous study demonstrated that during plant reproductive processes, intracellular NO is produced by pollen, and that such NO could be important in signalling interactions between pollen and stigma. The aim of this study was to establish whether pollen releases NO and nitrite, using a wide range of plant species. Using a fluorimetric assay in conjunction with electron paramagnetic resonance (EPR) spectroscopy, the present study demonstrated that all hydrating pollen examined released NO, although some appeared to have more activity than others. Additionally, gas phase ozone-based chemiluminescence data showed that nitrite is also released from hydrating pollen. Given that pollen has interactions with other cells, for example in allergenic rhinitis (hay fever) in humans, it suggests that NO might be involved in mediating the responses of both plant and animal cells to pollen. These findings may have important implications for future allergy research, as it is possible that pollen-derived NO and nitrite may impact on mammalian cells during pollen-induced allergic responses. (C) 2008 Elsevier Masson SAS. All rights reserved.