Studies on the formation of the influenza virus envelope.
Studies on the formation of the influenza virus envelope.
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流感病毒包膜形成的研究。
DOI：
10.1016/0042-6822(74)90335-3
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发表时间：
1974
期刊：
影响因子：
3.7
通讯作者：
A. Hay
中科院分区：
文献类型：
--
作者：
A. Hay
本文报道了将鸡瘟病毒特异性多肽插入感染的鸡细胞质膜并随后将其掺入病毒颗粒中的研究。病毒特异性的血凝素、神经氨酸酶糖多肽和基质多肽被结合到感染细胞的质膜中，尽管这些多肽在感染过程中积累成为质膜的主要成分，但由于病毒感染，任何宿主细胞膜蛋白都没有明显的移位。血凝素前体和神经氨酸酶多肽是在与粗面内质网紧密结合的情况下合成的，在与粗面内质网结合的同时至少部分糖基化。这些多肽随后通过平滑内质网转移到质膜上，在质膜上裂解前体血凝素多肽，得到两个血凝素多肽。对感染细胞质膜制剂的血凝组分和非血凝组分的分析表明，病毒血凝素分布在膜的离散区域，而神经氨酸酶则更普遍地分布在膜上。基质蛋白似乎是与质膜紧密结合而合成的，并直接插入含有病毒血凝素的质膜区域，这一过程被认为是病毒组装前包膜形成的最后阶段。
An investigation of the insertion of fowl plague virus-specified polypeptides into the plasma membrane of infected chick cells and their subsequent incorporation into virus particles is reported. Virus-specified haemagglutinin and neuraminidase glycopolypeptides and matrix polypeptide were incorporated into the plasma membrane of infected cells, and, although these polypeptides accumulated during infection to become major components of the plasma membrane, there was no apparent displacement of any host cell membrane protein as a result of virus infection. The haemagglutinin precursor and neuraminidase polypeptides were synthesized in close association with the rough endoplasmic reticulum and were at least partially glycosylated while associated with this structure. These polypeptides were subsequently transferred via the smooth endoplasmic reticulum to the plasma membrane where the precursor haemagglutinin polypeptide was cleaved to yield the two haemagglutinin polypeptides. Analyses of haemagglutinating and nonhaemagglutinating fractions of plasma membrane preparations from infected cells indicated that the virus haemagglutinin was localized in discrete regions of the membrane whereas the neuraminidase was more generally distributed throughout the membrane. The matrix protein appeared to be synthesized in close association with the plasma membrane and inserted directly into regions of the plasma membrane which contain virus haemagglutinin, and it is suggested that this process represents the final stage in the formation of the virus envelope prior to virus assembly.