LC-MS Identification, Isolation, and Structural Elucidation of Anti-HIV Tigliane Diterpenoids from Wikstroemia lamatsoensis
LC-MS Identification, Isolation, and Structural Elucidation of Anti-HIV Tigliane Diterpenoids from Wikstroemia lamatsoensis
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DOI：
10.1021/acs.jnatprod.1c00570
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发表时间：
2021-08-17
影响因子：
5.1
通讯作者：
Li, Wei
中科院分区：
文献类型：
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作者：
Zhang, Mi;Otsuki, Kouharu;Li, Wei
几十年来，结构多样的二萜类化合物吸引了人们对药物开发的浓厚研究兴趣。利用LC-MS分离分离技术，首次对拉马索维克斯特罗草属植物进行了化学成分研究，分离得到8个化合物(1-8)，其中包括两个新化合物：维克斯特罗菌素D(1)和维克斯特罗菌素E(2)。通过大量的物理化学和光谱分析，确定了新的结构。总结了太子油1-8的ESIMS/MS裂解特征。其中化合物8、5和7的抗HIV活性最强，其IC50值分别为0.18、3.8和12.8 nM。
Structurally diverse tigliane diterpenoids have drawn significant research interest for drug discovery over many decades. Using LC-MS-guided fractionation and separation, the first phytochemical investigation on Wikstroemia lamatsoensis led to the isolation of eight tiglianes (1-8), including two new compounds, wikstrocin D (1) and wikstrocin E (2). The new structures were elucidated based on extensive physicochemical and spectroscopic analyses. The characteristic ESIMS/MS fragmentations of tiglianes 1-8 were also summarized. Among the isolated tiglianes, three compounds (8, 5, and 7) showed the most potent anti-HIV activity, with IC50 values of 0.18, 3.8, and 12.8 nM, respectively.