S100 IS PRESENT IN DEVELOPING CHICKEN NEURONS AND SCHWANN-CELLS AND PROMOTES MOTOR-NEURON SURVIVAL INVIVO
S100 IS PRESENT IN DEVELOPING CHICKEN NEURONS AND SCHWANN-CELLS AND PROMOTES MOTOR-NEURON SURVIVAL INVIVO
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DOI：
10.1002/neu.480230410
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发表时间：
1992-06-01
期刊：
影响因子：
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通讯作者：
RATNER, N
中科院分区：
文献类型：
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作者：
BHATTACHARYYA, A;OPPENHEIM, RW;RATNER, N
我们使用识别 S100（一种在神经组织中高度富集的小型酸性蛋白）的多克隆抗血清对胚胎鸡腰骶脊髓和后肢切片进行染色。早在胚胎第 5 天 (E)，就在发育中的背根神经节 (DRG) 感觉神经元和腹侧脊髓运动神经元中检测到 S100 免疫反应性，并且染色在孵化期间持续存在。相比之下，S100 的表达首先在 E13 的雪旺细胞中变得明显，就在髓鞘形成之前，并且在发育中的皮肤或肌肉中未检测到。由于 S100-β 存在于运动和感觉神经元中，并且已知可在体外促进神经元存活和神经突延伸（Winningham-Major、Staecker、Barger、Coats 和 Van Eldik，1989），因此我们在卵内存活测定中测试了 S100 促进神经元存活的能力。在自然发生的运动神经元细胞死亡期间，向卵内鸡胚添加 S100 导致运动神经元存活率显着增加，但对 DRG 中感觉神经元的体内存活率没有影响。 S100 存在于脊髓运动神经元中，并且添加 S100 可以增强这些细胞在体内的存活率，这一发现与 S100 可能在发育过程中充当天然存在的神经元存活因子的可能性是一致的。
We used polyclonal antisera recognizing S100, a small acidic protein highly enriched in nervous tissue, to stain sections of embryonic chicken lumbosacral spinal cord and hindlimb. S100 immunoreactivity was detected in developing sensory neurons of the dorsal root ganglia (DRG) and motor neurons of the ventral spinal cord as early as embryonic day (E) 5, and staining persisted through hatching. In contrast, expression of S100 first became apparent in Schwann cells at E13, just before myelination, and was not detected in developing skin or muscle. Since S100-beta was present in motor and sensory neurons and is known to promote neuronal survival and neurite extension in vitro (Winningham-Major, Staecker, Barger, Coats, and Van Eldik, 1989), we tested the ability of S100 to promote neuron survival in an in ovo survival assay. Addition of S100 to chick embryos in ovo during the period of naturally occurring motor neuron cell death resulted in a significant increase in motor neuron survival, but had no effect on the in vivo survival of sensory neurons in the DRG. The findings that S100 is present in spinal motor neurons and that the addition of S100 enhances the survival of these cells in vivo are consistent with the possibility that S100 may act as a naturally occurring neuron survival factor during development.