Detection of enteroviral infection in paraffin-embedded tissue by the RNA polymerase chain reaction technique.
Detection of enteroviral infection in paraffin-embedded tissue by the RNA polymerase chain reaction technique.
复制标题
通过RNA聚合酶链式反应技术检测石蜡包埋组织中的肠道病毒感染。
DOI：
10.1093/ajcp/96.5.568
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发表时间：
1991
影响因子：
3.5
通讯作者：
Tycko,B
中科院分区：
文献类型：
--
作者：
Redline,RW;Genest,DR;Tycko,B
报道了一种使用 RNA 聚合酶链式反应技术，使用石蜡包埋组织块中的单链 RNA 肠道病毒回顾性诊断感染的方法。该方法利用肠道病毒基因组 5' 非翻译区的极端序列保守性，并使用嵌套寡核苷酸引物进行两轮扩增，从而无需使用放射性试剂即可快速诊断。在组织病理学评估后怀疑病毒感染的情况下，当新鲜或冷冻组织经常无法获得时，该技术应该被证明是有用的。该方法的敏感性通过从尸体解剖检查中获得的 4 岁肝组织中提取的肠道病毒 11 RNA 的成功扩增得到证明，该肝组织在死后 45 小时初步固定并包埋。
A method using the RNA polymerase chain reaction technique to diagnose infection retrospectively using single-stranded RNA enteroviruses in paraffin-embedded tissue blocks is reported. This method takes advantage of extreme sequence conservation in the 5' untranslated region of the enteroviral genome and uses two rounds of amplification with nested oligonucleotide primers, thus allowing rapid diagnosis without the use of radioactive reagents. The technique should prove useful in cases in which viral infection is suspected after histopathologic evaluation, when fresh or frozen tissues often are unavailable. The sensitivity of the method is demonstrated by successful amplification of Enterovirus 11 RNA extracted from 4-year-old liver tissue obtained at autopsy examination that was initially fixed and embedded 45 hours after death.