Dose Effects of Corticosteroids on the Development of Osteonecrosis in Rabbits
Dose Effects of Corticosteroids on the Development of Osteonecrosis in Rabbits
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DOI：
10.3899/jrheum.080324
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发表时间：
2008-12-01
影响因子：
3.9
通讯作者：
Iwamoto, Yukihide
中科院分区：
文献类型：
--
作者：
Motomura, Goro;Yamamoto, Takuaki;Iwamoto, Yukihide
Objective.皮质类固醇剂量与骨坏死（ON）患病率之间的关系尚未建立。我们在兔模型中研究了皮质类固醇对ON发展的剂量效应。将1（12只家兔）、5（12只家兔）、20（20只家兔）和40（25只家兔）mg/kg的醋酸甲泼尼龙（MPSL）一次性肌内注射至家兔右臀中肌。注射MPSL 4周后，取股骨和肱骨近端和远端进行组织病理学检查，观察ON的存在情况，并在注射皮质类固醇前后进行血液学检查。在1、5、20和40 mg/kg MPSL组家兔中，ON的发生率分别为0、42%、70%和96%。MPSL剂量与ON的发生率有显著相关性，组织学观察发现，MPSL 5 mg/kg组ON周围可见修复组织，而MPSL 20和40 mg/kg组ON周围未见修复组织。在血液学检查中，高脂血症和血小板减少症在40 mg/kg MPSL组最明显。本研究提示皮质激素的剂量在兔ON的发生发展中起重要作用。修复过程也被发现受到皮质类固醇剂量的影响。皮质类固醇诱导的高脂血症和血小板减少症似乎与ON的发生率相关（2008年10月1日首次发布; J Rheumol 2008;35：2395-9; doi：10.3899/ jrheum.080324）
Objective. The relationship between dose of corticosteroids and the prevalence of osteonecrosis (ON) has not been established. We examined the dose effects of corticosteroids on the development of ON in a rabbit model.Methods. Rabbits were injected once intramuscularly with 1 (12 rabbits), 5 (12 rabbits), 20 (20 rabbits), and 40 (25 rabbits) mg/kg of methylprednisolone acetate (MPSL) into the right gluteus medius muscle. Four weeks after the MPSL injection, the proximal and distal parts of both the femora and humeri were histopathologically examined for the presence of ON. Hematological examinations were performed before and after the corticosteroid injection.Results. In rabbits with 1, 5, 20, and 40 mg/kg MPSL, the incidence of ON was 0, 42%, 70%, and 96%, respectively. The dose of MPSL showed a significant association with the incidence of ON. Histologically, reparative tissues around the ON sites were observed in the rabbits with 5 mg/kg MPSL, but not observed in rabbits with 20 and 40 mg/kg MPSL. On hematological examination, hyperlipidemia and thrombocytopenia were most apparent in the rabbits receiving 40 mg/kg MPSL.Conclusion. The study suggested that the dose of corticosteroids plays an important role in the development of ON in rabbits. The repair process was also found to be influenced by the dose of corticosteroids. Corticosteroid-induced hyperlipidemia and thrombocytopenia seemed to be associated with the incidence of ON. (First Release Oct 1 2008; J Rheumatol 2008;35:2395-9; doi: 10.3899/ jrheum.080324)