Biochemical study of resistance to imidacloprid in B biotype Bemisia tabaci from Guatemala
Biochemical study of resistance to imidacloprid in B biotype Bemisia tabaci from Guatemala
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DOI：
10.1002/ps.649
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发表时间：
2003-03-01
影响因子：
4.1
通讯作者：
Toscano, NC
中科院分区：
文献类型：
--
作者：
Byrne, FJ;Castle, S;Toscano, NC
使用切除的棉花叶进行的系统吸收生物测定证实了危地马拉烟草粉虱烟粉虱Gennadius种群对吡虫啉的抗性。萘酯酶聚丙烯酰胺凝胶电泳鉴定为B型。从田间采集后，确定抗性是源自加州帝王谷的敏感菌株的58倍。抗性水平增加到126倍，在这个人口在其连续暴露于系统处理的棉花。在生化调查中，没有检测到的NADPH依赖的混合功能氧化酶代谢的C-14-吡虫啉在选择过程中的任何时间。相比之下，来自家蝇的微粒体制剂容易产生大量的母体化合物的单羟基和烯烃衍生物。家蝇MFO对吡虫啉的去甲作用可能是杀虫剂对这种昆虫的毒性低于粉虱的原因，尽管两种物种中吡虫啉与烟碱乙酰胆碱受体之间的结合特性相似。(C)2003化学工业协会。
Systemic uptake bioassays using excised cotton leaves confirmed resistance to imidacloprid in a Guatemalan population of the tobacco whitefly Bemisia tabaci Gennadius. Polyacrylamide gel electrophoresis of naphthyl esterases identified the insects as B-types. Upon collection from the field, resistance was determined to be 58-fold relative to a susceptible strain originating in the Imperial Valley of California. Resistance levels increased to 126-fold in this population during its continuous exposure to systemically treated cotton. In biochemical investigations, there was no detectable NADPH-dependent mixed function oxidase metabolism of C-14-imidacloprid at any time during the selection process. In contrast, microsomal preparations from housefly abdomens readily produced significant amounts of the mono-hydroxy and olefin derivatives of the parent compound. Detoxification of imidacloprid by housefly MFOs may account for reports of lower toxicity of the insecticide towards this insect compared with whiteflies, despite similar binding properties between imidacloprid and the nicotinic acetylcholine receptors in both species. (C) 2003 Society of Chemical Industry.