The protein that binds the 3' end of histone mRNA: A novel RNA-binding protein required for histone pre-mRNA processing
The protein that binds the 3' end of histone mRNA: A novel RNA-binding protein required for histone pre-mRNA processing
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DOI：
10.1101/gad.10.23.3028
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发表时间：
1996-12-01
影响因子：
10.5
通讯作者：
Marzluff, WF
中科院分区：
文献类型：
--
作者：
Wang, ZF;Whitfield, ML;Marzluff, WF
依赖复制的组蛋白mRNA没有多聚腺苷酸化，而是以一个包含茎环结构的保守的26核苷酸结构结尾。组蛋白mRNA的许多细胞周期调控是转录后的，并由组蛋白mRNA的3'端介导。结合组蛋白mRNA 3'端的茎环结合蛋白（SLBP）是参与大多数（如果不是全部）转录后调控事件的因子的候选者。我们利用最近开发的酵母三杂交系统从人、小鼠和青蛙中克隆了SLBP的cDNA。人SLBP是一种31 - kD的蛋白质，包含一个新的RNA结合结构域，该结构域已被定位到蛋白质的一个73个氨基酸区域。克隆的SLBP就是结合在组蛋白mRNA 3'端的蛋白质，因为针对SLBP的特异性抗体可去除核提取物和多聚核糖体提取物中的所有特异性结合活性。这些被去除相关成分的提取物不能有效地切割组蛋白前体mRNA，这表明SLBP是有效加工组蛋白前体mRNA所必需的。
Replication-dependent histone mRNAs are not polyadenylated but end in a conserved 26-nucleotide structure that contains a stem-loop. Much of the cell cycle regulation of histone mRNA is post-transcriptional and is mediated by the 3' end of histone mRNA. The stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA is a candidate for the factor that participates in most, if not all, of the post-transcriptional regulatory events. We have cloned the cDNA for the SLBP from humans, mice, and frogs, using the recently developed yeast three-hybrid system. The human SLBP is a 31-kD protein and contains a novel RNA-binding domain, which has been mapped to a 73-amino-acid region of the protein. The cloned SLBP is the protein bound to the 3' end of histone mRNA as antibodies specific for the SLBP remove all specific binding activity from nuclear and polyribosomal extracts. These depleted extracts do not cleave histone pre-mRNA efficiently, demonstrating that the SLBP is required for efficient histone pre-mRNA processing.