Differential regulation of CD4+ T helper cell responses by curcumin in experimental autoimmune encephalomyelitis
Differential regulation of CD4+ T helper cell responses by curcumin in experimental autoimmune encephalomyelitis
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DOI：
10.1016/j.jnutbio.2011.10.002
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发表时间：
2012-11-01
影响因子：
5.6
通讯作者：
Bright, John J.
中科院分区：
文献类型：
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作者：
Kanakasabai, Saravanan;Casalini, Eli;Bright, John J.
营养保健品和植物化学品是人类健康和疾病的重要调节剂。姜黄素是从植物姜黄（姜黄）的根茎中分离出的多酚植物化学物质，几个世纪以来一直被传统地用于治疗炎症和伤口愈合。系统分析表明，姜黄素通过抗氧化，抗增殖和抗炎特性发挥其有益作用。我们和其他人已经表明，姜黄素改善多发性硬化症的实验性自身免疫性脑脊髓炎（EAE）模型。在这项研究中，我们表明，C57 BL/6小鼠诱导发展EAE表达水平升高的干扰素（IFN）γ和白细胞介素（IL）-17在中枢神经系统（CNS）和淋巴器官，显着降低姜黄素在体内治疗后。EAE小鼠还显示IL-12和IL-23的表达升高，在姜黄素治疗后降低。用姜黄素进行离体和体外处理导致培养物中IFN γ、IL-17、IL-12和IL-23的分泌呈剂量依赖性降低。姜黄素对EAE的抑制作用还与CNS和淋巴器官中IL-10、过氧化物酶体增殖物激活受体γ和CD 4（+）CD 25（+）Foxp 3（+）Treg细胞的上调有关。这些发现强调了姜黄素对EAE中CD 4（+）T辅助细胞反应的差异调节。(C)2012 Elsevier Inc. All rights reserved.
Nutraceuticals and phytochemicals are important regulators of human health and diseases. Curcumin is a polyphenolic phytochemical isolated from the rhizome of the plant Curcuma longa (turmeric) that has been traditionally used for the treatment of inflammation and wound healing for centuries. Systematic analyses have shown that curcumin exerts its beneficial effects through antioxidant, antiproliferative and anti-inflammatory properties. We and others have shown earlier that curcumin ameliorates experimental autoimmune encephalomyelitis (EAE) model for multiple sclerosis. In this study, we show that C57BL/6 mice induced to develop EAE express elevated levels of interferon (IFN) gamma and interleukin (IL)-17 in the central nervous system (CNS) and lymphoid organs that decreased significantly following in vivo treatment with curcumin. The EAE mice also showed elevated expression of IL-12 and IL-23 that decreased after treatment with curcumin. Ex vivo and in vitro treatment with curcumin resulted in a dose-dependent decrease in the secretion of IFN gamma, IL-17, IL-12 and IL-23 in culture. The inhibition of EAE by curcumin was also associated with an up-regulation of IL-10, peroxisome proliferator activated receptor gamma and CD4(+)CD25(+) Foxp3(+) Treg cells in the CNS and lymphoid organs. These findings highlight that curcumin differentially regulates CD4(+) T helper cell responses in EAE. (C) 2012 Elsevier Inc. All rights reserved.