Reversion of autonomously replicating sequence mutations in Saccharomyces cerevisiae: creation of a eucaryotic replication origin within procaryotic vector DNA
Reversion of autonomously replicating sequence mutations in Saccharomyces cerevisiae: creation of a eucaryotic replication origin within procaryotic vector DNA
复制标题
酿酒酵母自主复制序列突变的逆转：在原核载体 DNA 内创建真核复制起点
DOI：
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复制
发表时间：
1990
影响因子：
5.3
通讯作者：
S. Kearsey
中科院分区：
文献类型：
--
作者：
David Kipling;S. Kearsey
为了研究有缺陷的复制子如何获得复制能力，我们研究了自主复制序列（ARS）突变的逆转。通过对缺乏功能性复制起点的酿酒酵母质粒进行诱变，我们获得了一系列赋予质粒ARS活性的顺式作用突变。原始质粒含有一个因点突变而失活的ARS元件，但令人惊讶的是，获得的10个独立的Ars+回复体中只有1个显示出该元件的回复突变。在其余的回复体中，M13 载体 DNA 中的序列变化会产生新的 ARS。在两种情况下，单个核苷酸的变化导致与 ARS 共识的匹配得到改善，而其他 6 个案例则显示载体序列的小重复，从而与 ARS 共识产生了额外的匹配。这些结果表明，复制起点分布的变化可能是通过点突变而不是通过预先存在的起点序列的转座而从头出现的。
To investigate how a defective replicon might acquire replication competence, we have studied the reversion of autonomously replicating sequence (ARS) mutations. By mutagenesis of a Saccharomyces cerevisiae plasmid lacking a functional origin of replication, we have obtained a series of cis-acting mutations which confer ARS activity on the plasmid. The original plasmid contained an ARS element inactivated by point mutation, but surprisingly only 1 of the 10 independent Ars+ revertants obtained shows a back mutation in this element. In the remainder of the revertants, sequence changes in the M13 vector DNA generate new ARSs. In two cases, a single nucleotide change results in an improved match to the ARS consensus, while six other cases show small duplications of vector sequence creating additional matches to the ARS consensus. These results suggest that changes in replication origin distribution may arise de novo by point mutation rather than by transposition of preexisting origin sequences.
DNA 在 lambda 复制起点弯曲的直接证据。
DOI：
10.1126/science.2951850
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Zahn,K;Blattner,FR
通讯作者：
Blattner,FR
影响酿酒酵母自主复制序列 ARS1 的缺失突变。
影响因子：
5.3
作者：
Celniker,SE;Sweder,K;Srienc,F;Bailey,JE;Campbell,JL
通讯作者：
Campbell,JL
涉及染色体维护的 DNA 结构的计算机建模。
影响因子：
14.9
作者：
Eckdahl,TT;Anderson,JN
通讯作者：
Anderson,JN