Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development.
Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development.
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发表时间：
2005
影响因子：
5.3
通讯作者：
Yoshikazu Nakamura;Y. Hamada;T. Fujiwara;H. Enomoto;T. Hiroe;Satoshi Tanaka;M. Nose;M. Nakahara;N. Yoshida;T. Takenawa;K. Fukami
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文献类型：
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作者：
Yoshikazu Nakamura;Y. Hamada;T. Fujiwara;H. Enomoto;T. Hiroe;Satoshi Tanaka;M. Nose;M. Nakahara;N. Yoshida;T. Takenawa;K. Fukami
磷酸肌肽特异性磷脂酶C （PLC）是磷酸化肌肽转化的关键酶，参与多种生理功能。我们分析了PLCdelta1基因敲除小鼠，发现PLCdelta1是维持皮肤稳态所必需的。然而，在PLCdelta1基因敲除小鼠中，尽管PLCdelta1基因广泛分布，但除了脱发和跑步外，没有其他显著异常。在这里，我们报道了缺乏PLCdelta1和PLCdelta3的小鼠在胚胎第11.5天（E11.5）至E13.5天死亡。PLCdelta1/PLCdelta3双敲除小鼠胎盘正常迷宫结构严重破坏，胎盘血管化减少，迷宫区滋养细胞增殖和凋亡异常。此外，通过四倍体聚集法提供正常胎盘的PLCdelta1/PLCdelta3双敲除胚胎在E14.5后存活，清楚地表明胚胎致命性是由滋养层细胞缺陷引起的。基于这些结果，我们得出结论，PLCdelta1和PLCdelta3在滋养细胞中对胎盘发育至关重要。
Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in phosphoinositide turnover and is involved in a variety of physiological functions. We analyzed PLCdelta1 knockout mice and found that PLCdelta1 is required for the maintenance of skin homeostasis. However, there were no remarkable abnormalities except hair loss and runting in PLCdelta1 knockout mice, even though PLCdelta1 is broadly distributed. Here, we report that mice lacking both PLCdelta1 and PLCdelta3 died at embryonic day 11.5 (E11.5) to E13.5. PLCdelta1/PLCdelta3 double-knockout mice exhibited severe disruption of the normal labyrinth architecture in the placenta and decreased placental vascularization, as well as abnormal proliferation and apoptosis of trophoblasts in the labyrinth area. Furthermore, PLCdelta1/PLCdelta3 double-knockout embryos supplied with a normal placenta by the tetraploid aggregation method survived beyond E14.5, clearly indicating that the embryonic lethality is caused by a defect in trophoblasts. On the basis of these results, we conclude that PLCdelta1 and PLCdelta3 are essential in trophoblasts for placental development.