Sequence structures of two developmentally regulated, alternative DNA deletion junctions in Tetrahymena thermophila.
Sequence structures of two developmentally regulated, alternative DNA deletion junctions in Tetrahymena thermophila.
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嗜热四膜虫中两个发育调控的选择性 DNA 缺失连接的序列结构。
DOI：
10.1128/mcb.8.9.3947-3950.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Yao,MC
中科院分区：
文献类型：
--
作者：
Austerberry,CF;Yao,MC
已知特定DNA序列的缺失发生在嗜热膜内，这是一个发育调节的过程。特定区域（区域M）的缺失先前显示有两种可选的大小，0.6或0.9千碱基（kb） （C.F. Austerberry， C.D. Allis和M. c . c .）。姚，法官。学会科学。美国81:7383-7387)。在这项研究中，确定了两个缺失的核苷酸序列。这两个缺失共享相同的右连接，但它们的左连接相距0.3 kb。一个8碱基对（bp）序列存在于0.6 kb缺失的两个连接处，但在0.9 kb缺失的左侧连接处仅存在5 bp的直接重复序列。进一步比较发现，在两个左连接点附近有一个共同的10-bp序列，在右连接点附近有一个类似的反向序列。这些序列可能在缺失过程的发育调控中发挥作用。
Deletions of specific DNA sequences are known to occur inTetrahymena thermophilaas a developmentally regulated process. Deletions of a particular region (region M) were previously shown to be of two alternative sizes, 0.6 or 0.9 kilobases (kb) (C.F. Austerberry, C.D. Allis, and M.-C. Yao, Proc. Natl. Acad. Sci. USA 81: 7383-7387). In this study, the nucleotide sequences for both deletions were determined. These two deletions share the same right junction, but their left junctions are 0.3 kb apart. An 8-base-pair (bp) sequence is present at both junctions of the 0.6-kb deletion, but only 5 bp of this direct repeat are present at the left junction of the 0.9-kb deletion. Further comparison revealed a common 10-bp sequence near each of the two left junctions and a similar sequence in inverted orientation near the right junction. These sequences may play a role in the developmental regulation of the deletion process.