Why is the molecular identification of the forensically important blowfly species Lucilia caesar and L. illustris (family Calliphoridae) so problematic?
Why is the molecular identification of the forensically important blowfly species Lucilia caesar and L. illustris (family Calliphoridae) so problematic?
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DOI：
10.1016/j.forsciint.2012.08.020
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发表时间：
2012-11-30
影响因子：
2.2
通讯作者：
Desmyter, Stijn
中科院分区：
文献类型：
--
作者：
Sonet, Gontran;Jordaens, Kurt;Desmyter, Stijn
绿蝇属（Lucilia）蝇种常用于法医学调查，以估计死亡时间（PMI）。两个近缘种Lucilia caesar和L.插图画家很难辨认。以往的研究表明，线粒体细胞色素c氧化酶亚基I（COI）标记可用于鉴定许多种绿蝇。然而，对于L.凯撒和L. illustris由于一些欧洲标本显示相同的单倍型。在这里，我们调查了58个新的欧洲雄性L. illustris和L.检测其形态学鉴定，并对其COI片段进行测序。此外，其他两个线粒体（细胞色素c氧化酶亚基II和16 S）和两个核（内部转录间隔区2和28 S核糖体RNA）标记，获得了这些样品的一个子集。对于每个标记，每个物种内的遗传分歧与物种之间的遗传分歧在相同的范围内，证实了两个物种之间的密切关系。此外，对于每个基因片段，两个物种共享至少一个单倍型/基因型。因此，没有一个被测试的分子标记可以单独或组合使用，以区分L。illustris和L.凯撒(c)2012爱思唯尔爱尔兰有限公司保留所有权利。
Species of the fly genus Lucilia are commonly used in forensic investigations to estimate the postmortem interval (PMI). Two close-related species Lucilia caesar and L. illustris are difficult to identify. Previous studies showed that the mitochondrial cytochrome c oxidase subunit I (COI) marker could be used to identify many Lucilia species. However, mixed results were obtained for L. caesar and L. illustris due to some European specimens showing identical haplotypes. Here, we investigated 58 new European male specimens of L. illustris and L. caesar whose morphological identifications were checked and for which COI fragments were sequenced. In addition, two other mitochondrial (cytochrome c oxidase subunit II and 16S) and two nuclear (internal transcribed spacer 2 and 28S ribosomal RNA) markers were obtained for a subset of these samples. For each marker, genetic divergence within each species was in the same range as between species, confirming the close relationship between both species. Moreover, for each of the gene fragments, both species shared at least one haplotype/genotype. Hence, none of the molecular markers tested could be used, alone or in combination, to discriminate between L. illustris and L. caesar. (c) 2012 Elsevier Ireland Ltd. All rights reserved.