Spontaneous mutation rates in cloned murine tumors do not correlate with metastatic potential, whereas the prevalence of karyotypic abnormalities in the parental tumors does.
Spontaneous mutation rates in cloned murine tumors do not correlate with metastatic potential, whereas the prevalence of karyotypic abnormalities in the parental tumors does.
复制标题
克隆鼠肿瘤的自发突变率与转移潜力无关，而亲代肿瘤中核型异常的发生率却与转移潜力相关。
DOI：
10.1002/ijc.2910400321
复制
发表时间：
1987
影响因子：
6.4
通讯作者：
Frost,P
中科院分区：
文献类型：
--
作者：
Kendal,WS;Wang,RY;Frost,P
我们测试了这样的假设：高度恶性的细胞系在基因组上比恶性程度较低的细胞系更不稳定，并且这种不稳定性在克隆中比在细胞系中更明显。我们将 MDAY-D2 与其非转移性变体 D36W25 进行了比较，涉及 (I) 平行克隆内哇巴因耐药性的发生率和 (2) G 带核型异常的患病率。我们检测到哇巴因抗性的自发突变率之间没有显着差异。然而，MDAY-D2 细胞系具有更高的染色体异常患病率和更大的多样性。对这些看似不一致的结果的一种可能解释是，基因组不稳定性可能在整个肿瘤进展过程中保持基本恒定，而遗传变化的积累可能是观察到的异常发生率增加和进展过程中选择性生存优势发展的原因。
We tested the hypothesis that highly malignant cell lines are genomically more unstable than their less malignant counterparts, and that this instability is more pronounced in clones than in cell lines. We compared MDAY‐D2 to its non‐metastatic variant, D36W25, with regard to (I) the rate of development of ouabain resistance within parallel clones and (2) the prevalence of G‐banded karyotypic abnormalities. We detected no significant difference between the spontaneous mutation rates for ouabain resistance. However, the MDAY‐D2 cell line possessed both a higher prevalence and greater diversity of chromosomal abnormalities. One possible explanation for these seemingly inconsistent results is that genomic instability may remain essentially constant throughout tumor progression, whereas an accumulation of genetic changes may be responsible for the observed increased prevalence of abnormalities and the development of selective survival advantages during progression.