Antioxidant responses of triangle sail mussel Hyriopsis cumingii exposed to toxic Microcystis aeruginosa and thermal stress
Antioxidant responses of triangle sail mussel Hyriopsis cumingii exposed to toxic Microcystis aeruginosa and thermal stress
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三角帆蚌有毒铜绿微囊藻和热应激的抗氧化反应
DOI：
10.1016/j.scitotenv.2020.140754
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发表时间：
2020-11-15
影响因子：
9.8
通讯作者：
Sui, Yanming
中科院分区：
文献类型：
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作者：
Liu, Yimeng;Yang, Min;Sui, Yanming
随着气候变化，有害藻华（HAB）和热应激在全球水生态系统中变得更加普遍，特别是在富营养化栖息地。在这里，我们的研究考察了形成蓝藻的铜绿微囊藻和热应激对生态重要物种三角帆蚌抗氧化反应的综合影响。一系列酶的不同反应，例如在接触有毒蓝藻铜绿假单胞菌14天和7天净化期间，分析了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST），以及参与抗氧化防御机制的信号代谢物，包括活性氧（ROS）、丙二醛（MDA）和谷胱甘肽（GSH）。铜绿假单胞菌暴露后，三角藻中SOD和GPx活性以及ROS和MDA含量增加，而CAT活性降低。热应激导致CAT减少，GSH积累，GST和SOD增加。同时，除GST活性外，大多数参数也观察到铜绿假单胞菌、热应激和时间之间的交互作用。帆蚌中微囊藻毒素（MC）的总量随着暴露的铜绿微囊藻浓度的增加而增加，与是否存在热应激无关。虽然贻贝中约 50% 的 MC 在净化期间下降，但大多数参数因蓝藻暴露和热应激而发生变化。总的来说，这些发现表明有毒蓝藻或热应激会诱导氧化应激，并分别严重影响与抗氧化防御机制相关的酶活性和中间体水平。更重要的是，它们的组合可能会加剧对帆蚌的毒性影响。 (C) 2020 Elsevier B.V. 保留所有权利。
Harmful algal blooms (HABs) and thermal stress as climate changes become more common in global water ecosystem, especially under eutrophic habitats. Here our study examined the combined impacts of bloom forming cyanobacteria Microcystis aeruginosa and thermal stress on the antioxidant responses of the ecologically important species triangle sail mussel Hyriopsis cumingii. The differential responses of a series of enzymes, e.g. superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST), as well as signal metabolites including reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH) involved in antioxidant defense mechanisms were analyzed during 14 d exposure to toxic cyanobacterium M. aeruginosa and 7 d depuration period. The activities of SOD and GPx as well as the content of ROS and MDA in H. cumingii increased, while CAT activity reduced due to M. aeruginosa exposure. Thermal stress resulted in decrease of CAT, the accumulation of GSH and the enhance of GST and SOD. Meanwhile, the interactive effects among M. aeruginosa, thermal stress and time were also observed on most parameters except for GST activity. The total amount of microcystins (MC) in sail mussels increased with concentrations of exposed M. aeruginosa, independently of the presence or absence of thermal stress. Although around 50% of MC in mussels dropped in the depuration period, most parameters showed alterations because of cyanobacteria exposure and thermal stress. Overall, these findings suggested that toxic cyanobacteria or thermal stress induces oxidative stress and severely affects the enzymes activities and intermediates level associated with antioxidant defense mechanisms in sail mussels respectively. More importantly, the toxic impacts on sail mussels could be intensified by their combination. (C) 2020 Elsevier B.V. All rights reserved.