Brain 2-Arachidonoylglycerol Levels Are Dramatically and Rapidly Increased Under Acute Ischemia-Injury Which Is Prevented by Microwave Irradiation.
Brain 2-Arachidonoylglycerol Levels Are Dramatically and Rapidly Increased Under Acute Ischemia-Injury Which Is Prevented by Microwave Irradiation.
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DOI：
10.1007/s11745-016-4144-y
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发表时间：
2016-04
期刊：
影响因子：
1.9
通讯作者：
Golovko MY
中科院分区：
文献类型：
--
作者：
Brose SA;Golovko SA;Golovko MY
脑2-花生四烯酰甘油（2-AG）参与许多关键的生理和病理生理调节机制，凸显了准确测定脑2-AG的重要性。在本研究中，我们验证了头部聚焦微波辐射（MW）作为一种方法，以防止死后大脑2-AG的变化前分析。我们比较了MW与冷冻以防止2-AG诱导，并估计了暴露于MW后的外源性和内源性2-AG稳定性。使用MW，我们已经测量，第一次，真正的2-AG脑水平在基础条件下，30秒后，从颅骨脑切除，并在暴露于5分钟的脑全缺血。我们的数据表明，脑2-AG水平是瞬间和显着增加约60倍后，脑从颅骨移除。脑全脑缺血5分钟后，2-AG水平也增加了3.5倍，但不太显著。我们的数据表明，脑组织固定MW是一个必要的技术，以测量真实的基础2-AG水平和2-AG的变化，在不同的实验条件下，包括全球缺血，2-AG是稳定的，暴露于MW。
The involvement of brain 2-arachidonoylglycerol (2-AG) in a number of critical physiological and pathophysiological regulatory mechanisms highlights the importance for an accurate brain 2-AG determination. In the present study, we validated head-focused microwave irradiation (MW) as a method to prevent postmortem brain 2-AG alterations before analysis. We have compared MW to freezing to prevent 2-AG induction and estimated exogenous and endogenous 2-AG stability upon exposure to MW. Using MW, we have measured, for the first time, true 2-AG brain levels under basal conditions, 30 sec after brain removal from the cranium, and upon exposure to 5 min of brain global ischemia. Our data indicate that brain 2-AG levels are instantaneously and dramatically increased ~ 60 fold upon brain removal from the cranium. Upon 5 min of brain global ischemia 2-AG levels are also, but less dramatically, increased 3.5 fold. Our data indicate that brain tissue fixation with MW is a required technique to measure both true basal 2-AG levels and 2-AG alterations under different experimental conditions including global ischemia, and 2-AG is stable upon exposure to MW.