Resonance Raman Spectra of Protohemin and Protohemin-Imidazole Complex,
Resonance Raman Spectra of Protohemin and Protohemin-Imidazole Complex,
复制标题
原血红素和原血红素-咪唑复合物的共振拉曼光谱，
DOI：
--
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
H. Bernstein
中科院分区：
文献类型：
--
作者：
A. L. Verma;H. Bernstein
Resonance Raman spectra of aqueous solutions of protohemin and its complex with imidazole have been obtained using several exciting lines from Ar+ and HeCd lasers. The effects of different axial ligands to iron in hemin are reflected in the resonance Raman spectra of their respective complexes. Within experimental limits, hemin does not show any anomalously polarized bands (Q1 > ¾) while its complex with imidazole shows several such bands when excitation is in the α and β absorption regions. When excitation is in the Soret region, a polarized band ∼ 1500 cm−1 appears in the spectra of hemin-imidazole complex and heme proteins. It is proposed as a spin state marker as well as to characterize the position of the iron ion relative to the porphyrin plane.
Excitation profiles for some of the bands of hemin confirm the assigment of the α band near 5300 A in the unresolved visible absorption spectrum.
