PROTEIN-PROTEIN INTERACTIONS AND 5'-SPLICE-SITE RECOGNITION IN MAMMALIAN MESSENGER-RNA PRECURSORS
PROTEIN-PROTEIN INTERACTIONS AND 5'-SPLICE-SITE RECOGNITION IN MAMMALIAN MESSENGER-RNA PRECURSORS
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DOI：
10.1038/368119a0
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发表时间：
1994-03-10
期刊：
影响因子：
64.8
通讯作者：
MANLEY, JL
中科院分区：
文献类型：
--
作者：
KOHTZ, JD;JAMISON, SF;MANLEY, JL
在复杂前体信使RNA的加工过程中，具体的剪接位点是如何被识别的还不清楚。小的核核糖核蛋白颗粒（snRNP）也参与其中，但其本身不足以定义剪接位点。现在，一种对体外剪接至关重要的人类蛋白质，称为选择性剪接因子/剪接因子2，显示出与U1 snRNP颗粒在结合前mRNA中合作。这种合作可能是通过剪接因子的富含精氨酸/丝氨酸的结构域与U1 snRNP特异性蛋白中的类似区域之间的特异性相互作用实现的。
Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.