High stromal Foxp3-positive T cell number combined to tumor stage improved prognosis in head and neck squamous cell carcinoma
High stromal Foxp3-positive T cell number combined to tumor stage improved prognosis in head and neck squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2017.02.023
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发表时间：
2017-04-01
期刊：
影响因子：
4.8
通讯作者：
Saussez, Sven
中科院分区：
文献类型：
--
作者：
Kindt, Nadege;Descamps, Geraldine;Saussez, Sven
目的：头颈部鳞状细胞癌（HNSCC）是世界上最常见的癌症之一，主要由炎性免疫细胞浸润。我们的目的是评估Foxp3+ T细胞在HNSCC的数量，报告其预后的权力相比，其他riskfactor.Material和methods：我们的临床系列是由21个无肿瘤的癌周上皮细胞，49低度异型增生，43高度异型增生和110个癌样本，包括HPV感染的一些情况下。结果：Foxp3+ T细胞的浸润随肿瘤从正常上皮、异型增生到癌变的进展而增加，HPV阳性者Foxp3+ T细胞的浸润较HPV阴性者更为明显。动物实验表明，E7癌蛋白表达与肿瘤中Foxp3+ T细胞募集的增加、肿瘤发病的延迟和动物存活率的提高显著相关。单变量考克斯回归分析表明，间质区室中Foxp3+ T细胞数量高与患者无复发生存期和总生存期较长相关。Foxp3+ T细胞数量提高了肿瘤分期的预后价值。多变量分析报告说，基质Foxp3+ T细胞数量是一个强大的预后因素独立的经典的危险因素，如烟草，酒精，和HPV的status.Conclusion：Foxp3+ T细胞数量是一个重要的预后因素HNSCC，提高肿瘤分期，和病毒E7可能发挥作用的Foxp3+ T细胞浸润到肿瘤。(C)2017爱思唯尔有限公司版权所有
Objectives: Head and neck squamous cell carcinomas (HNSCC), one of the most frequent cancers in the world, are largely infiltrated by inflammatory immune cells. Our aim was to evaluate the number of Foxp3+ T cells in HNSCC, reporting its prognostic power in comparison to other risk factors.Material and methods: Our clinical series was composed of 21 tumor-free peri-tumoral epithelia, 49 low grade dysplasia, 43 high grade dysplasia and 110 carcinoma samples including some cases with HPV infection. In vivo experiments were conducted on 80 C3 H/HeN mice which were orthotopically injected with SCCVII CT, E7, E6 and E6/E7 cell lines.Results: Foxp3+ T cell infiltration increased with tumor progression from normal epithelia, dysplasia to carcinoma and the increase is more important in HPV+ patients than in negative ones. Animal experiments revealed that E7 oncoprotein expression was significantly associated with an increase in Foxp3+ T cell recruitment in tumor, a delay in tumor onset and improved animal survival. Univariate Cox regression analyses demonstrated that high Foxp3+ T cell number in stromal compartment is associated with longer patient recurrence-free and overall survivals. Foxp3+ T cell number improved the prognostic value of tumor stage. Multivariate analyses reported that stromal Foxp3+ T cell number is a strong prognostic factor independent of classical risk factors such as tobacco, alcohol, and HPV status.Conclusion: Foxp3+ T cell number is a significant prognostic factor for HNSCC, improving the tumor stage, and that viral E7 may play a role in the Foxp3+ T cell infiltration to the tumor. (C) 2017 Elsevier Ltd. All rights reserved.