Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis
Role of ClpP in biofilm formation and virulence of Staphylococcus epidermidis
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ClpP 在表皮葡萄球菌生物膜形成和毒力中的作用
DOI：
10.1016/j.micinf.2007.06.012
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发表时间：
2007-09-01
影响因子：
5.8
通讯作者：
Gao, Qian
中科院分区：
文献类型：
--
作者：
Wang, Chongzhen;Li, Min;Gao, Qian
由院内主要病原体表皮葡萄球菌引起的感染以植入性医疗器械上形成生物膜为特征。然而，生物膜形成的分子基础及其调控还不完全清楚。在这里，我们描述了ClpP蛋白酶在表皮葡萄球菌生物膜发育和毒力中的重要作用。我们构建了一株形成生物被膜的表皮葡萄球菌临床分离株的ClpP等基因突变株。突变菌株在体外显示生物膜形成减少，并在生物膜相关感染的大鼠模型中降低毒力。在载体上表达ClpP可以恢复突变菌株的生物被膜形成能力，但引入ClpP基因的催化失活等位基因则不能恢复该突变菌株的生物膜形成能力。这些观察结果表明，ClpP的多肽酶功能决定了它在生物膜形成中的作用。本工作的实验数据还表明，ClpP影响细菌在塑料表面的初始附着，这是生物膜形成的第一步。此外，ClpP被发现受群体感应AGR的调节，这表明前面描述的AGR对塑料表面初始附着的影响的一部分可能是由ClpP介导的。(C)2007年爱思唯尔·马森公司。版权所有。
Infections caused by the leading nosocomial pathogen Staphylococcus epidermidis are characterized by biofilm formation on implanted medical devices. However, the molecular basis of biofilm formation and its regulation are not completely understood. Here, we describe an important role of the ClpP protease in biofilm development and virulence of S. epidermidis. We constructed an isogenic clpP mutant strain of a biofilm-forming clinical isolate of S. epidermidis. The mutant strain showed decreased biofilm formation in vitro and reduced virulence in a rat model of biofilm-associated infection. Biofilm forming ability of the mutant strain could be restored by expressing clpP on a plasmid, but not when a catalytically inactive allele of clpP gene was introduced. These observations indicate that the peptidase function of ClpP determines its role in biofilm formation. Experimental data in this work also suggested that clpP influenced initial attachment of bacteria on the plastic surface, the first step of biofilm formation. Furthermore, clpP was found to be regulated by the quorum-sensing agr, suggesting that part of the previously described influence of agr on the initial attachment to plastic surfaces may be mediated by clpP. (C) 2007 Elsevier Masson SAS. All rights reserved.