The association of Tyro3/Axl/Mer signaling with inflammatory response, disease activity in patients with primary Sjogren's syndrome
The association of Tyro3/Axl/Mer signaling with inflammatory response, disease activity in patients with primary Sjogren's syndrome
复制标题
Tyro3/Axl/Mer信号传导与原发性干燥综合征患者炎症反应、疾病活动的关系
DOI：
10.1016/j.jbspin.2015.01.008
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发表时间：
2015-07-01
期刊：
影响因子：
4.2
通讯作者：
Zhong, Renqian
中科院分区：
文献类型：
--
作者：
Qin, Baodong;Wang, Jiaqi;Zhong, Renqian
目的：Tyro 3/Axl/Mer信号传导的缺陷可能导致凋亡细胞的吞噬作用受损，最终导致自身免疫性疾病的发展。TAM信号传导与几种自身免疫性疾病的相关性已被研究，但在原发性干燥综合征中仍不清楚。因此，本研究的目的是评估原发性干燥综合征中TAM信号水平及其临床意义。研究方法：采用实时荧光定量聚合酶链反应（Real-Time Polymerase Chain Reaction，RT-PCR）检测43例pSS和46例对照组外周血单核细胞中Mer、Tyro-3、Axl、Gas 6和蛋白S的mRNA表达。采用酶联免疫吸附试验（ELISA）检测血浆中可溶性TAM信号水平，并分析其与临床特征的关系。结果：pSS患者外周血Tyro-3、Axl mRNA表达水平降低。当考虑血浆水平时，观察到可溶性Mer水平升高，具有统计学显著性差异。可溶性Mer水平与IgG水平（r = 0.53，P < 0.01）、红细胞沉降率水平（r = 0.44，P < 0.01）和干燥综合征疾病活动指数（r = 0.48，P< 0.01）呈正相关。有SSA/SSB者血清可溶性Mer水平高于无SSA/SSB者。结论：pSS患者血浆sMer水平升高，与炎症反应和疾病活动有关。(C)2015年法国rhumatologie协会。由Elsevier Masson SAS出版。All rights reserved.
Objectives: Defects in Tyro3/Axl/Mer signaling may lead to impaired phagocytosis of apoptotic cells, eventually contributing to the development of autoimmune disease. The association of TAM signaling with several autoimmune disease has been investigated, but it remains unclear in primary Sjogren's syndrome. Therefore, the aim of this study was to evaluate the level of TAM signaling in primary Sjogren's syndrome with its clinical significance. Methods: Real-Time Polymerase Chain Reaction was used to determine the mRNA expression of Mer, Tyro-3, Axl, Gas6, and Protein S in Peripheral Blood Mononuclear Cell from 43 pSS and 46 control. The Enzyme-Linked Immunosorbent Assay method was used to test plasma levels of soluble TAM signaling from those individuals, and the relationship of their levels with clinical characteristic was evaluated. Results: The mRNA expression levels of Tyro-3, Axl were decreased in pSS patients. When considering the plasma level, increased levels of soluble Mer was observed with statistically significant difference. Soluble Mer levels were positively correlated with IgG levels (r = 0.53, P < 0.01), Erythrocyte Sedimentation Rate levels (r = 0.44, P < 0.01) and Sjogren's Syndrome Disease Activity Index (r = 0.48, P< 0.01). And the levels of soluble Mer in patients with the presence of SSA/SSB were higher than those without SSA/SSB. Conclusions: The plasma levels of sMer were increased in pSS patients, which was associated with inflammatory response and disease activity. (C) 2015 Societe francaise de rhumatologie. Published by Elsevier Masson SAS. All rights reserved.