Histopathological and immunohistochemical characterization of canine prostate cancer
Histopathological and immunohistochemical characterization of canine prostate cancer
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DOI：
10.1002/pros.20720
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发表时间：
2008-04-01
期刊：
影响因子：
2.8
通讯作者：
Teske, Erik
中科院分区：
文献类型：
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作者：
Lai, Chen-Li;van den Ham, Rene;Teske, Erik
背景。在这项研究中，我们试图通过对肿瘤组织学亚型进行分类来确定犬前列腺癌（cPC）的起源，并将这些亚型与几种组织特异性和分化标记物的组合表达特征相关联。方法。对cPC进行组织形态学检查，并通过免疫组织化学检测细胞角蛋白标记物CK14、HMWCK、CK5、CK18和CK7以及标记物UPIII、PSA和PSMA。结果。在组织病理学上，可以区分六种生长模式。最常见的模式是实性、筛状和微乳头状生长模式，而肉瘤样、小腺泡/导管和管状乳头状生长模式则不太常见。坚固的生长模式在阉割的狗中更为常见（P = 0.027）。免疫组织化学显示，约一半的cPC病例显示PSA（8/20）和PSMA（10/20）的表达； 85%和60%的cPC表达UPIII (17/20)和CK7 (12/20)，而13和12 cPC分别表达CK5和CK14；所有cPC均表达CK18。 CK14 在实体生长模式中的表达频率显着高于微乳头状模式和筛状模式，而 UPIII 的表达频率则低于微乳头状模式。结论。与最常见的人类前列腺癌相比，犬前列腺癌似乎更具侵袭性且分化程度较低。将 cPC 中标记物的表达模式与正常犬前列腺组织中的标记物的表达模式进行比较，cPC 最有可能源自集合管而不是外周腺泡。还考虑到犬前列腺癌对雄激素戒断疗法没有反应，犬前列腺癌大多类似于人类、雄激素难治性、低分化前列腺癌。
BACKGROUND. In this study we try to identify the origin of canine prostate cancer (cPC) by classifying the tumors histological subtypes and relate these subtypes to their combined expressional characteristics of several tissue specific and differentiation markers.METHODS. cPCs were examined histomorphologically and by immunohistochemical detection of the cytokeratin markers CK14, HMWCK, CK5, CK18, and CK7, and of the markers UPIII, PSA and PSMA.RESULTS. Histopathologically, six growth patterns could be differentiated. The most frequent patterns were solid, cribriform and micropapillary growth patterns, while sarcomatoid, small acinar/ductal, and tubulo-papillary growth patterns were less frequent present. Solid growth patterns were significantly (P = 0.027) more often seen in castrated dogs. Immunohistochemically, about half of the cPC cases showed expression of PSA (8/20) and PSMA (10/20); 85% and 60% of the cPC expressed UPIII (17/20) and CK7 (12/20), while 13 and 12 cPC expressed CK5 and CK14, respectively; all cPC expressed CK18. CK14 was significantly more often and UPIII less frequent expressed in the solid growth patterns than in the micropapillary and cribriform patterns, respectively.CONCLUSIONS. Canine prostate cancer appear to be more aggressive and of a less differentiated type than most common human prostate cancers. Comparing the expression patterns of the markers in cPC to those in normal canine prostate tissue, cPC most likely originates from the collecting ducts rather than from the peripheral acini. Given also the fact that canine prostate cancer is unresponsive to androgen withdrawal therapy, canine prostate cancer mostly resembles human, androgen refractory, poorly differentiated prostate cancer.