Temporal and spatial expression of a position specific antigen, AV-1, in chick limb buds.
Temporal and spatial expression of a position specific antigen, AV-1, in chick limb buds.
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位置特异性抗原 AV-1 在鸡肢芽中的时间和空间表达。
DOI：
10.1016/0012-1606(88)90341-7
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发表时间：
1988
影响因子：
2.7
通讯作者：
T. Momoi
中科院分区：
文献类型：
--
作者：
K. Ohsugi;H. Ide;T. Momoi
在以前的研究中，我们证明了存在的位置特异性抗原（AV-1）在鸡肢芽在早期发育阶段。在这里，我们报告的时间和空间的表达和生化特性的AV-1抗原。间接免疫荧光染色和免疫印迹分析清楚地表明，AV-1抗原是一种糖蛋白，定位于质膜上，它是从阶段19和高度表达在阶段22-26中的一些中远端到前远端区的肢芽。在翅芽，在阶段28，AV-1抗原微弱地检测到之间的限制空间的前软骨区的桡骨和尺骨，和掌骨2和3，但不是掌骨3和4。AV-1抗原在肢芽中的这种阶段特异性和“位置特异性”表达强烈表明AV-1抗原或含有它的细胞参与了肢型形成的决定。
In a previous study, we demonstrated the presence of a position-specific antigen (AV-1) in chick limb buds at an early developmental stage. Here, we reported the temporal and spatial expressions and the biochemical characterization of the AV-1 antigen. Indirect immunofluorescence staining and immunoblot analysis clearly showed that the AV-1 antigen is a glycoprotein that is localized on the plasma membrane and that it is expressed from stage 19 and highly expressed at stages 22–26 in some middle-distal to anterior-distal region of limb buds. In the wing bud, at stage 28, the AV-1 antigen was faintly detected in the restricted space between the precartilaginous regions of the radius and the ulna, and those of the metacarpals 2 and 3, but not those of the metacarpals 3 and 4. Such stage-specific and “position-specific” expressions of the AV-1 antigen in limb buds strongly suggest that the AV-1 antigen or cells containing it are involved in determination of the limb pattern formation.
胚胎发育过程中纤连蛋白分布的时空模式。
DOI：
--
发表时间：
1981
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
Melnick,M;Jaskoll,T;Brownell,AG;MacDougall,M;Bessem,C;Slavkin,HC
通讯作者：
Slavkin,HC