ADSORPTION OF IMMUNOGLOBULIN-G, PROTEIN-A, AND FIBRONECTIN IN THE SUBMONOLAYER REGION EVALUATED BY A COMBINED STUDY OF ELLIPSOMETRY AND RADIOTRACER TECHNIQUES
ADSORPTION OF IMMUNOGLOBULIN-G, PROTEIN-A, AND FIBRONECTIN IN THE SUBMONOLAYER REGION EVALUATED BY A COMBINED STUDY OF ELLIPSOMETRY AND RADIOTRACER TECHNIQUES
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DOI：
10.1016/0021-9797(85)90115-8
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发表时间：
1985-01-01
影响因子：
9.9
通讯作者：
RONNBERG, I
中科院分区：
文献类型：
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作者：
JONSSON, U;MALMQVIST, M;RONNBERG, I
在一项应用椭偏仪和放射性示踪剂技术的研究中，对免疫球蛋白G、蛋白A和纤维连接蛋白的吸附进行了定量。不同浓度的~(14)C和~(125)I标记的蛋白质被吸附在具有3种不同固体表面张力的二氧化硅表面。还研究了抗体与预吸附抗原膜的相互作用。用椭偏仪、接触角测量和扫描电子显微镜对其表面进行了表征。用凝胶电泳法对蛋白质进行了鉴定。免疫球蛋白G和蛋白A的椭圆偏振系数与吸附总量呈线性关系，纤维连接蛋白在两种表面上的吸附偏离了线性。结果表明，在不同类型的表面上吸附的IgG膜的结构相似，而吸附的HFN膜的结构与固体表面张力有关。根据亚单层吸附分子的椭圆偏振理论对实验结果进行了讨论。从放射性标记实验获得的吸附蛋白质的数量与根据椭圆偏振数据计算的结果吻合得很好。
The adsorption of the proteins: immunoglobulin G, protein A and fibronectin was quantitated in a study applying both ellipsometry and radiotracer techniques. 14C- and 125I-labeled proteins at different concentrations were adsorbed to silica surfaces with 3 different solid surface tensions. The interactions of antibodies with a preadsorbed antigen film was also investigated. The surfaces were characterized by ellipsometry, contact angle measurements and scanning electron microscopy. The proteins were characterized by gel electrophoresis. A linear relationship for the change in the ellipsometric parameter .DELTA. and adsorbed amount was found for immunoglobulin G and protein A. A deviation from linearity was found for fibronectin adsorbed to 2 types of surfaces. The result was interpreted as similarities in the structure of the adsorbed IgG film on the different types of surfaces, whereas the structure of the adsorbed HFN film showed dependence on the solid surface tension. The results were discussed in view of the ellipsometric theories for adsorbed molecules in the submonolayer region. The amounts of adsorbed proteins obtained from the radiolabeling experiments agreed well with those calculated from ellipsometric data.