"Thymineless" death in androgen-independent prostatic cancer cells.
"Thymineless" death in androgen-independent prostatic cancer cells.
复制标题
不依赖雄激素的前列腺癌细胞中的“无胸腺嘧啶”死亡。
DOI：
10.1016/0006-291x(89)91035-8
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Isaacs,JT
中科院分区：
文献类型：
--
作者：
Kyprianou,N;Isaacs,JT
The molecular mechanism of “thymineless” death induced by 5-fluoro-deoxyuridine or trifluorothymidine, in androgen-independent rat prostatic adenocarcinoma AT-3 cells was investigated. Fragmentation of genomic DNA into discrete multiples of a nucleosomal unit (i.e. 180bp subunit) and induction of expression of TRPM-2, a programmed cell death-associated gene, temporally correlated with the activation of programmed cell death in this system. In contrast, killing of AT-3 cells by osmotic lysis, or membrane-targeted metabolic inhibitors results in neither the stereotypic DNA fragmentation into nucleosomal oligomers nor the elevation of TRPM-2 mRNA levels but to non-specific biochemical changes characteristic of necrosis. These results suggest that androgen-independent prostatic cancer cells retain a major portion of the programmed cell death cascade which can be activated by non-androgen ablative cytotoxic drugs that induce “thymineless” death.
