Soybean 14-3-3 gene family: identification and molecular characterization
Soybean 14-3-3 gene family: identification and molecular characterization
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DOI：
10.1007/s00425-010-1315-6
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发表时间：
2011-03-01
期刊：
影响因子：
4.3
通讯作者：
Dhaubhadel, Sangeeta
中科院分区：
文献类型：
--
作者：
Li, Xuyan;Dhaubhadel, Sangeeta
14-3-3s是一组在真核生物中普遍存在的蛋白质。植物14-3-3蛋白是由一个多基因家族编码的蛋白质，参与植物生长发育和逆境保护的信号转导途径。最近在拟南芥和水稻中的研究已经证明了14-3-3s的异构体特异性及其客户蛋白相互作用。然而，14-3-3基因家族在豆科植物中的详细特征尚未报道。在这项研究中，大豆14-3-3蛋白进行了鉴定和分子特征。对大豆基因组和表达序列标签数据库进行数据挖掘，共鉴定出18个14-3-3基因，其中16个是转录基因。所有16个SGF 14在胚组织中具有较高的表达，表明它们在种子发育中的潜在作用。所有转录的SGF 14的亚细胞定位表明，14-3-3蛋白在大豆中具有亚型特异性，然而，也观察到一些重叠之间密切相关的亚型。与拟南芥和水稻14-3-3s的比较分析表明，SGF 14 s也分为拟南芥和非拟南芥两类。然而，与拟南芥和水稻14-3-3s不同，SGF 14 s只包含属于每个类别的一种基因结构。总的来说，大豆由迄今为止表征的最大的14-3-3蛋白质家族组成。我们的研究结果为进一步研究SGF 14的作用及其参与豆类特异性功能提供了坚实的框架。
The 14-3-3s are a group of proteins that are ubiquitously found in eukaryotes. Plant 14-3-3 proteins are encoded by a large multigene family and are involved in signaling pathways to regulate plant development and protection from stress. Recent studies in Arabidopsis and rice have demonstrated the isoform specificity in 14-3-3s and their client protein interactions. However, detailed characterization of 14-3-3 gene family in legumes has not been reported. In this study, soybean 14-3-3 proteins were identified and their molecular characterization performed. Data mining of soybean genome and expressed sequence tag databases identified 18 14-3-3 genes, of them 16 are transcribed. All 16 SGF14s have higher expression in embryo tissues suggesting their potential role in seed development. Subcellular localization of all transcribed SGF14s demonstrated that 14-3-3 proteins in soybean have isoform specificity, however, some overlaps were also observed between closely related isoforms. A comparative analysis of SGF14s with Arabidopsis and rice 14-3-3s indicated that SGF14s also group into epsilon and non-epsilon classes. However, unlike Arabidopsis and rice 14-3-3s, SGF14s contained only one kind of gene structure belonging to each class. Overall, soybean consists of the largest family of 14-3-3 proteins characterized to date. Our results provide a solid framework for further investigations into the role of SGF14s and their involvement in legume-specific functions.