Specific sequence selection and next generation resequencing of 68 E-coli genes using HybSelect™
Specific sequence selection and next generation resequencing of 68 E-coli genes using HybSelect™
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DOI：
10.1016/j.nbt.2009.08.013
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发表时间：
2009-11-30
影响因子：
5.4
通讯作者：
Beier, Markus
中科院分区：
文献类型：
--
作者：
Schracke, Nadine;Kornmeyer, Tobias;Beier, Markus
近年来，下一代测序技术取得了巨大进展，允许以高度成本效益的方式进行基因组测序。然而，这些测序仪的样品制备仍然是一个瓶颈，因为人类基因组过于复杂，无法进行常规重新测序。我们在这里提出了一个深入的研究HybSelect（TM），一种方法，可以特异性地丰富了大量的基因或区域的任何染色体DNA的兴趣。该研究使用大肠杆菌K12 MG 1655作为模型生物，以检测方法保真度、容量或重现性等参数，作为原理验证。
Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect (TM), a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.