BACTERIOPHAGE-RESISTANT MUTANTS OF BACILLUS-THURINGIENSIS WITH DECREASED VIRULENCE IN PUPAE OF HYALOPHORA-CECROPIA
BACTERIOPHAGE-RESISTANT MUTANTS OF BACILLUS-THURINGIENSIS WITH DECREASED VIRULENCE IN PUPAE OF HYALOPHORA-CECROPIA
复制标题
DOI：
10.1128/jb.167.1.18-24.1986
复制
发表时间：
1986-07-01
影响因子：
3.2
通讯作者：
BOMAN, HG
中科院分区：
文献类型：
--
作者：
HEIERSON, A;SIDEN, I;BOMAN, HG
从苏云金芽孢杆菌的晶体阴性亲本菌株开始，我们分离出某些噬菌体抗性突变体，该突变体在天蚕蛾（Hyalophora cecropia）蛹中表现出毒力降低。这些菌株（I 类突变体）具有高度多效性，对七八种不同的噬菌体表现出抗性，对甲氧西林敏感，并且鞭毛缺失。他们在体外对天蚕免疫血淋巴也更敏感。此外，至少三种蛋白质的出口减少。回复体（II 类突变体）对噬菌体敏感、有毒并且对青霉素衍生物具有抗性。一种 II 类突变体在所有检查的特性中都是完全回复突变体。另一个 II 类突变体是不完全回复体，仍然对免疫同淋巴敏感并且蛋白质输出受到抑制。毒力与噬菌体抗性本身或鞭毛的缺乏无关，因为影响这些特性的其他突变体是有毒的。可以排除的毒力原因的其他因素是细胞外蛋白酶和溶血素的产生。
Starting from a crystal-negative parental strain of Bacillus thuringiensis, we isolated certain bacteriophage-resistant mutants which showed decreased virulence in pupae of the cecropia moth (Hyalophora cecropia). These strains (class I mutants) were highly pleiotropic and showed resistance to seven or eight different phages, sensitivity to methicillin, and loss of flagella. They were also more sensitive to cecropia immune hemolymph in vitro. In addition, the export of at least three proteins was reduced. Revertants (class II mutants) were sensitive to phages, virulent, and resistant to penicillin derivatives. One class II mutant was a complete revertant in all properties examined. The other class II mutant was an incomplete revertant still susceptible to immune homolymph and with repressed export of proteins. Virulence was not coupled to phage resistance as such or to lack of flagella because other mutants affected in these properties were virulent. Other factors which could be excluded as causes of virulence were production of extracellular protease and hemolysin.