Enamel Proteases in Secretory and Maturation Enamel of Rats Ingesting 0 and 100 ppm Fluoride in Drinking Water
Enamel Proteases in Secretory and Maturation Enamel of Rats Ingesting 0 and 100 ppm Fluoride in Drinking Water
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摄入饮用水中 0 和 100 ppm 氟化物的大鼠牙釉质分泌和成熟过程中的牙釉质蛋白酶
DOI：
10.1177/08959374890030022001
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发表时间：
1989
影响因子：
--
通讯作者：
L. M. Heffernan
中科院分区：
文献类型：
--
作者：
Pamela DenBesten;L. M. Heffernan
在饮用水中摄入高氟形成的牙釉质在成熟期的有机含量增加，这可能是由于釉质形成的早期成熟阶段釉质原蛋白的分解延迟所致。氟化牙釉质中釉原蛋白分解的延迟表明氟对牙釉质蛋白酶的影响，而牙釉质蛋白是早期分泌的牙釉质蛋白的水解酶。在这项研究中，我们比较了氟化牙釉质和对照分泌期和成熟期牙釉质中的蛋白水解酶。牙釉质在含0.1%明胶的十二烷基硫酸钠凝胶中脱矿和分离。凝胶在10 0 mm ol/L Tris-HCI，pH 8，10 m ol/L CaCl2中孵育后，考马斯蓝染色，可见清晰的降解明胶带。氟化牙釉质和对照牙釉质的蛋白水解酶活性条带相似。成熟期比釉质形成分泌期的蛋白水解酶多。与对照成熟期牙釉质相比，氟化成熟期牙釉质中的几种蛋白酶对明胶底物的消化较少。这提示氟化成熟期牙釉质中蛋白水解酶的分泌量或活性可能发生改变。
Dental enamel formed during ingestion of high levels of fluoride in drinking water has an increased organic content in the maturation stage, which may be due to a delay in the breakdown of amelogenins during the early-maturation stage of enamel formation. This delay in the breakdown of amelogenins in fluorosed enamel suggests an effect of fluoride on enamel proteases which hydrolyze the early secreted enamel proteins. In this study, we compared the proteases present in fluorosed and control secretory-stage and maturation-stage enamel. Enamel was demineralized and separated in SDS gels containing 0.1% gelatin. After incubation in 100 mmol/L Tris-HCI, pH 8, with 10 mmol/L CaCl2, the gels were stained with Coomassie Blue, and proteases were seen as clear zones of degraded gelatin. Similar bands of proteolytic activity were seen in fluorosed and in control enamel. In the maturation stage, more proteases were present than in the secretory stage of enamel formation. Less digestion of gelatin substrate occurred in several proteases found in the fluorosed maturation-stage enamel as compared with the control maturation-stage enamel. This suggests that the amount of protease secreted or the activity of the proteases may be altered in fluorosed maturation-stage enamel.