Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography
Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography
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DOI：
10.1016/j.infrared.2011.11.004
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发表时间：
2012-01-01
影响因子：
3.3
通讯作者：
Saito, Kozo
中科院分区：
文献类型：
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作者：
Salaimeh, Ahmad A.;Campion, Jeffrey J.;Saito, Kozo
我们以前表明，红外热成像（IRT）可以用来量化活大肠杆菌，一个代表性的革兰氏阴性菌，在液体生长培养基。在这里，我们评估了IRT计数可行的代表性革兰氏阳性微生物金黄色葡萄球菌的能力。我们发现培养基的能量含量（EC）与测量的S活菌计数呈强正相关（r = 0.999）。金黄色葡萄球菌的范围从85个菌落形成单位（CFU）/ml到类似于4 × 10（8）CFU/ml。S. aureus的活性比E. colt在可比的细胞浓度表明IRT可用于区分属。由爱思唯尔公司出版
We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia colt, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to similar to 4 x 10(8) CFU/ml. The EC of S. aureus was similar to 2-fold higher than that of E. colt at comparable cell concentrations suggesting that IRT may be used to distinguish genera. Published by Elsevier B.V.