Enzyme- Facilitated Transport and Separation of Organic Acids through Liquid Membranes
Enzyme- Facilitated Transport and Separation of Organic Acids through Liquid Membranes
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酶促进有机酸通过液膜的运输和分离
DOI：
10.1021/ja00160a065
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发表时间：
1990
影响因子：
15
通讯作者：
J. Dordick
中科院分区：
文献类型：
--
作者：
D. Rethwisch;A. Subramanian;Gao Yi;J. Dordick
有机酸的选择性分离对于许多生物功能分子的生产是重要的，包括氨基酸、肽、脂肪和药物。1酶，特别是脂肪酶和蛋白酶，已被用于选择性地分离各种有机酸;然而，通常需要多个步骤。2此外，碳酸酐酶已被用于多种用于运输CO2的液膜中。3.在本研究中，我们将酶的选择性与液膜耦合，提供了一种选择性分离和纯化有机酸的一步法。我们的实验策略是使用所需的有机酸通过液膜的促进运输。这通过所需有机酸与有机液膜中包含的疏水醇的脂肪酶催化的酯化来进行（参见图1A）。所得酯分配到有机相中或水解成母体酸。一旦在有机相中，酯扩散穿过膜，其中第二脂肪酶催化酯水解成醇和母体酸。如果酶促途径明显快于有机酸通过膜的运输，这将导致所需酸的选择性纯化。用于这些研究的系统如图1B所示。（1）（a）汉森，P.E.;摩根，B。a.在肽：分析，合成，生物学：Udenfriend，S. Meinhofer，J.，编辑;出版社：Orlando。佛罗里达州，1984年。(b)Pirkle，WH; Doherty，E. MJ Am. 1989，III，4113 - 4114。(c)Kaiser，E. T.; Kezdy，F. J. Science 1984，226，249 - 255. (d)Wolfe，S.; Demain，AL;詹森，SE;韦斯特莱克，D. W. S. Science 1984，226，1386 - 1392. (e)Maccillon，A. R. JAOCS 1983，60，243A-246A。（2）（a）马特森，S. L.的;奎因，J. A. Annals NY Acad. sci. 1986，469，152 - 165. (b)奇巴塔岛; Tosa，T.;高田岛趋势Biotechnol. 1983，1，9 - 11.（3）（a）唐纳森，T. L.的;奎因，J. A.化学工程科学1975，30，103 - 115。(b)真的S.的R.;舒尔茨，JS Biochim。Biophys. Acta 1974，352，412 - 440。
The selective separation of organic acids is important for the production of numerous biologially functional molecules, including amino acids, peptides, fats, and pharmaceuticals. 1 Enzymes, specifically lipases and proteases, have been used to selectively separate a variety of organic acids; however, multiple steps are usually required. 2 In addition, carbonic anhydrase has been used in a variety of liquid membranes for transport of C02. 3 In the present study, we have coupled the selectivity of enzymes with liquid membranes to provide a single-step method to selectively separate and purify organic acids. Our experimental strategy was to use facilitative transport of a desired organic acid through a liquid membrane. This was carried out by the lipase-catalyzed esterification of the desired organic acid with a hydrophobic alcohol contained in an organic liquid membrane (see Figure 1 A). The resulting ester partitions into the organic phase or is hydrolyzed to the parent acid. Once in the organic phase, the ester diffuses across the membrane where a second lipase catalyzes ester hydrolysis into the alcohol and the parent acid. If the enzyme-facilitated pathway is significantly faster than transport of the organic acids through the membrane, this will result in selective purification of the desired acid. The system employed for these studies is shown inFigure IB.(1)(a) Hansen, P. E.; Morgan, B. A. In The Peptides: Analysis, Syn-thesis, Biology: Udenfriend, S.. Meinhofer, J., Eds.; Academic Press: Orlando. FL, 1984.(b) Pirkle, WH; Doherty, E. MJ Am. Chem. Soc. 1989, III, 4113-4114.(c) Kaiser, E. T.; Kezdy, F. J. Science 1984, 226, 249-255.(d) Wolfe, S.; Demain, AL; Jensen, SE; Westlake, D. W. S. Science 1984, 226, 1386-1392.(e) Macrae, A. R. JAOCS 1983, 60, 243A-246A.(2)(a) Matson, S. L.; Quinn, J. A. Annals NY Acad. Sci. 1986, 469, 152-165.(b) Chibata, I.; Tosa, T.; Takata, I. Trends. Biotechnol. 1983,/, 9-11.(3)(a) Donaldson, T. L.; Quinn, J. A. Chem. Eng. Sci. 1975, 30, 103-115.(b) Suchdeo. S. R.; Schultz, JS Biochim. Biophys. Acta 1974, 352,412-440.