Tetrahedral Framework Nucleic Acids Promote Senile Osteoporotic Fracture Repair by Enhancing Osteogenesis and Angiogenesis of Callus
Tetrahedral Framework Nucleic Acids Promote Senile Osteoporotic Fracture Repair by Enhancing Osteogenesis and Angiogenesis of Callus
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DOI：
10.1021/acsami.3c03569
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发表时间：
2023-05-18
影响因子：
9.5
通讯作者：
Luo，En
中科院分区：
文献类型：
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作者：
Liu，Li-Nan;Hu，Pei;Luo，En
老年性骨质疏松性骨折因其高发病率和高死亡率而日益受到重视。然而，到目前为止，还没有有效的治疗方法。老年性骨质疏松症以成骨和血管生成障碍为特征，促进成骨和血管生成也可促进骨质疏松性骨折的修复。四面体骨架核酸（tFNAs）是近年来广泛应用于生物医学领域的一种多功能纳米材料，可促进体外成骨和血管生成。因此，我们将tFNAs分别应用于完整和股骨骨折的老年骨质疏松症小鼠，从愈合早期骨痂的成骨和血管生成方面评价tFNAs对老年骨质疏松症和骨质疏松性骨折修复的影响，并初步探讨其可能的机制。结果表明，tFNA治疗后3周内对完整老年骨质疏松小鼠股骨和下颌骨的成骨和血管生成无明显影响，但tFNA可促进骨质疏松骨折修复中骨痂的成骨和血管生成，这可能是通过FoxO1相关的SIRT1通路调节的。结论：tFNAs可通过促进成骨和血管生成促进老年性骨质疏松性骨折的修复，为老年性骨质疏松性骨折的治疗提供了新的策略。
Senile osteoporotic fracture has aroused increasing attention due to high morbidity and mortality. However, to date, there is no effective therapeutic approach available. Senile osteoporosis is characterized by impaired osteogenesis and angiogenesis, osteoporotic fracture repair could also be promoted by enhancing osteogenesis and angiogenesis. Tetrahedral framework nucleic acids (tFNAs) are a multifunctional nanomaterial that have recently been extensively used in biomedical fields, which could enhance osteogenesis and angiogenesis in vitro. Therefore, we applied tFNAs to intact and femoral fractural senile osteoporotic mice, respectively, to evaluate the effects of tFNAs on senile osteoporosis and osteoporotic fracture repair regarding the osteogenesis and angiogenesis of the callus at the early healing stages and to initially explore the potential mechanism. The outcomes showed that tFNAs had no significant effects on the osteogenesis and angiogenesis of the femur and mandible in intact senile osteoporotic mice within 3 weeks after tFNA treatment, while tFNAs could promote osteogenesis and angiogenesis of callus in osteoporotic fracture repair, which may be regulated by a FoxO1-related SIRT1 pathway. In conclusion, tFNAs could promote senile osteoporotic fracture repair by enhancing osteogenesis and angiogenesis, offering a new strategy for the treatment of senile osteoporotic fracture.