Intra- and extracellular element concentrations of rat renal papilla in antidiuresis.
Intra- and extracellular element concentrations of rat renal papilla in antidiuresis.
复制标题
抗利尿作用中大鼠肾乳头的细胞内和细胞外元素浓度。
作者：
F. Beck;A. Dörge;R. Rick;K. Thurau
用电子探针分析法测定了大鼠肾乳头尖端不同细胞内和细胞外室中的元素浓度。尿浓度（平均值+/- SEM）为：尿素，1509 +/- 116;钾，268 +/- 32;钠，62 +/- 19 mmol X 1（-1）;渗透压，2548 +/- 141 mOsm X kg-1。间隙空间中的电解质浓度为：钠，437 +/- 19;氯，438 +/- 20;和钾，35 +/- 2毫摩尔X kg-1湿重。直血管血浆中的元素浓度几乎相同。乳头状集合管细胞中的值为：钠，28 +/- 1;氯，76 +/- 3;钾，135 +/- 3;和磷，316 +/- 7 mmol X kg-1湿重。在乳头状上皮细胞中观察到类似浓度。在间质细胞中，钾和磷的浓度几乎相同的集合管细胞，而钠和氯的浓度高约30毫摩尔× kg-1湿重。因此，各种乳头细胞的元素组成与近端肾小管细胞的元素组成没有实质性差异。这一发现表明，细胞内电解质的积累不是乳头状细胞适应高环境渗透压和氯化钠浓度的调节机制。
The element concentrations in various intra- and extracellular compartments of the tip of the rat renal papilla were determined during antidiuresis using electron microprobe analysis. Urinary concentrations (means +/- SEM) were: urea, 1509 +/- 116; potassium, 268 +/- 32; sodium, 62 +/- 19 mmoles X 1(-1); and osmolality, 2548 +/- 141 mOsm X kg-1. Electrolyte concentrations in the interstitial space were: sodium, 437 +/- 19; chloride, 438 +/- 20; and potassium, 35 +/- 2 mmoles X kg-1 wet wt. The vasa recta plasma exhibited almost identical element concentrations. The values in the papillary collecting duct cells were: sodium, 28 +/- 1; chloride, 76 +/- 3; potassium, 135 +/- 3; and phosphorus, 316 +/- 7 mmoles X kg-1 wet wt. Similar concentrations were observed in the papillary epithelial cells. In interstitial cells potassium and phosphorus concentrations were virtually identical to those of the collecting duct cells, whereas sodium and chloride concentrations were higher by about 30 mmoles X kg-1 wet wt. The element composition of the various papillary cells is, thus, not substantially different from that of proximal tubular cells. This finding demonstrates that cellular accumulation of electrolytes is not the regulatory mechanism by which papillary cells adapt osmotically to their high environmental osmolality and sodium chloride concentration.