Time-resolved Raman detection of .nu.(Fe-O) in an early intermediate in the reduction of oxygen by cytochrome oxidase
Time-resolved Raman detection of .nu.(Fe-O) in an early intermediate in the reduction of oxygen by cytochrome oxidase
复制标题
细胞色素氧化酶还原氧的早期中间体中 .nu.(Fe-O) 的时间分辨拉曼检测
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
G. Babcock
中科院分区：
文献类型：
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作者：
C. Varotsis;W. Woodruff;G. Babcock
Cytochrome oxidase contains four redox-active centers per functional unit. These reducing equivalents are transferred to the binuclear cyt a{sub 3}{hor ellipsis}Cu{sub B} center, which binds O{sub 2} and reduces it to H{sub 2}O. Although the reaction between O{sub 2} and cytochrome oxidase occurs too quickly to be studied by conventional stopped-flow techniques, Gibson and Greenwood showed that photolysis of the cytochrome a{sub 3}{sup 2+}-CO complex of the enzyme in the presence of O{sub 2} could be used to circumvent this limitation. The authors have developed techniques that allow them to carry out low-frequency Raman detection under flow/flash conditions; here they report v(Fe{sup 2+}-O) for an early intermediate in the cytochrome oxidase/dioxygen reaction.
Cytochrome oxidase contains four redox-active centers per functional unit. These reducing equivalents are transferred to the binuclear cyt a{sub 3}{hor ellipsis}Cu{sub B} center, which binds O{sub 2} and reduces it to H{sub 2}O. Although the reaction between O{sub 2} and cytochrome oxidase occurs too quickly to be studied by conventional stopped-flow techniques, Gibson and Greenwood showed that photolysis of the cytochrome a{sub 3}{sup 2+}-CO complex of the enzyme in the presence of O{sub 2} could be used to circumvent this limitation. The authors have developed techniques that allow them to carry out low-frequency Raman detection under flow/flash conditions; here they report v(Fe{sup 2+}-O) for an early intermediate in the cytochrome oxidase/dioxygen reaction.