Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles.
Preparation and cytotoxicity of chitosan-based hydrogels modified with silver nanoparticles.
复制标题
银纳米粒子修饰的壳聚糖基水凝胶的制备及其细胞毒性。
DOI：
10.1016/j.colsurfb.2017.09.044
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
A. Sobczak
中科院分区：
文献类型：
--
作者：
B. Tyliszczak;A. Drabczyk;S. Kudłacik;K. Bialik;R. Kijkowska;A. Sobczak
壳聚糖基水凝胶通常应用于医学和制药的各个领域。使用纳米银颗粒对水凝胶聚合物进行改性可导致形成具有增强的抗菌性能的材料。本文介绍了以壳聚糖为基质，银纳米粒子修饰的水凝胶材料的合成。首先，制备和表征的银纳米粒子使用紫外可见光谱已被证明。研究了纳米银改性水凝胶在蒸馏水和模拟体液中的溶胀性能和体外溶胀性能。此外，评价了对金黄色葡萄球菌和粪肠球菌的抗菌性能，以及通过XTT和MTT试验使用真皮细胞BJ（CRL-2522 TM）进行的用银纳米颗粒修饰的水凝胶材料的细胞毒性结果。
Chitosan based hydrogels are commonly applied in various fields of medicine and pharmacy. Modification of hydrogel polymers using nanosilver particles may result in formation of materials with enhanced antibacterial properties. In this article synthesis of hydrogel materials based on chitosan and modified with silver nanoparticles is presented. First, preparation and characterization of silver nanoparticles using UV–vis spectroscopy has been shown. Hydrogels modified with nanosilver particles were subjected to the measurements of swelling ability andin vitrotests in distilled water and Simulated Body Fluid (SBF), respectively. Additionally, evaluation of antibacterial properties againstStaphylococcus aureusandEnterococcus faecalisas well as results of cytotoxicity of hydrogel materials modified with silver nanoparticles conducted by means of XTT and MTT assays using dermis cells BJ (CRL-2522TM) have been presented.