Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms
Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms
复制标题
DOI：
10.1128/aem.66.2.836-838.2000
复制
发表时间：
2000-02-01
影响因子：
4.4
通讯作者：
Hassett, DJ
中科院分区：
文献类型：
--
作者：
Stewart, PS;Roe, F;Hassett, DJ
过氧化氢渗透到由野生型和过氧化氢酶缺陷型铜绿假单胞菌菌株形成的生物膜中使用微电极测量。流动的过氧化氢流（50 mM，1 h）不能穿透或杀死野生型生物膜，但穿透并部分杀死由其中katA基因被敲除的同基因菌株形成的生物膜。过氧化氢酶通过防止过氧化氢完全渗透到生物膜中来保护聚集的细菌。
The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.