Physicochemical characterization, digestion profile and gut microbiota regulation activity of intracellular polysaccharides from Chlorella zofingiensis.
Physicochemical characterization, digestion profile and gut microbiota regulation activity of intracellular polysaccharides from Chlorella zofingiensis.
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DOI：
10.1016/j.ijbiomac.2023.126881
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发表时间：
2023-09
影响因子：
8.2
通讯作者：
Peng Wan;Han Liu;Mengyan Ding;Kailu Zhang;Zhen Shang;Yuanli Wang;Yanli Ma
中科院分区：
文献类型：
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作者：
Peng Wan;Han Liu;Mengyan Ding;Kailu Zhang;Zhen Shang;Yuanli Wang;Yanli Ma
许多研究表明，微藻多糖具有多种生物活性，但其可消化性及其对人体肠道菌群的影响尚不清楚。本研究建立了模拟消化发酵系统，研究了青绿球藻（chlorellazofingiensis, CZIP-S3）胞内多糖的消化率和发酵情况。结果表明，CZIP-S3是一种由甘露糖、葡萄糖、半乳糖和鼠李糖组成的大分子多糖，由一个主链和两个支链重复单元组成。ccip - s3不能在上消化道消化。然而，cip - s3可以被肠道微生物代谢成更小的分子。发酵过程中pH值不断降低，短链脂肪酸含量稳步增加。此外，cip - s3还能调节肠道菌群的组成，降低厚壁菌门/拟杆菌门的比例，增加拟杆菌门、双歧杆菌门和akkermansia的相对丰度。这些数据表明，cip - s3可能被用作功能食品或益生元的成分，通过提高有益菌的相对丰度来改善人类健康。
A number of studies have shown that the polysaccharides from microalgae exhibit diverse biological activities, however, little is known about their digestibility and impact on human gut microbiota. In this study, a simulating digestion and fermentation system were established to investigate the digestibility and fermentation of intracellular polysaccharides fromChlorellazofingiensis(CZIP-S3). The results indicated that CZIP-S3 is a macromolecular polysaccharide composed of mannose, glucose, galactose and rhamnose, consisting of a main chain and two branched repeating units. CZIP-S3 could not be digested in the upper gastrointestinal tract. However, CZIP-S3 could be metabolized into smaller molecules by the gut microbiota. The pH values continuously decrease during fermentation, whereas, the amount of short-chain fatty acids steadily increase. Furthermore, CZIP-S3 could modulate the composition of gut microbiota,vialowering the ratio of Firmicutes/Bacteroidetes and increasing the relative abundance of Bacteroides, Bifidobacterium andAkkermansia. The data suggested that CZIP-S3 could potentially be used as an ingredient for functional foods or prebiotics to improve human health by promoting the relative abundances of beneficial bacteria.