Endurance Exercise Training Enhances Local Sex Steroidogenesis in Skeletal Muscle
Endurance Exercise Training Enhances Local Sex Steroidogenesis in Skeletal Muscle
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DOI：
10.1249/mss.0b013e31821e9d74
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发表时间：
2011-11-01
期刊：
影响因子：
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通讯作者：
Akimoto, Takayuki
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文献类型：
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作者：
Aizawa, Katsuji;Iemitsu, Motoyuki;Akimoto, Takayuki
AIZAWA, K.、M. IEMITSU、S. Maeda、N. MESAKI、T. USHIDA 和 T. AKIMOTO。耐力运动训练增强骨骼肌局部性类固醇生成。医学。科学。体育锻炼，卷。 43，第 11 期，第 2072-2080 页，2011 年。目的：耐力训练可改善骨骼肌功能，包括能量代谢和结构。性类固醇激素在一定程度上有助于运动引起的肌肉适应。最近，我们证明骨骼肌含有类固醇生成转换酶来合成性类固醇激素，并且急性耐力运动会激活骨骼肌中的局部类固醇生成。然而，长期耐力训练是否会导致骨骼肌类固醇生成增强尚不清楚。在这里，我们检查了长期耐力运动训练后骨骼肌中类固醇生成酶和性类固醇激素的变化。方法：将 11 只雄性大鼠分为两组：久坐组（n = 6）和训练组（n = 5）。在跑步机上进行耐力训练（30 m.min(-1)，30 分钟），持续 5 d.wk(-1)，持续 12 周。最后一次运动训练后48小时进行训练后收获。结果：训练后大鼠骨骼肌中3β-HSD、芳香酶细胞色素P450、5α-还原酶mRNA表达量显着高于久坐大鼠（P < 0.05）。训练后大鼠骨骼肌中芳香化酶细胞色素P450和5α-还原酶蛋白表达量也显着高于久坐大鼠（P < 0.05）。训练后大鼠骨骼肌中的肌肉二氢睾酮（DHT）浓度显着高于静坐大鼠（P < 0.01），但脱氢表雄酮、总睾酮、游离睾酮和雌二醇没有变化。此外，根据受训大鼠体重校正的肌肉重量与受训大鼠肌肉 DHT 浓度水平呈中度相关（r = 0.41，P < 0.05）。结论：耐力运动训练通过大鼠骨骼肌中的5α还原酶提高肌肉DHT浓度，提示局部生物活性雄激素代谢可能参与运动训练诱导的骨骼肌适应。
AIZAWA, K., M. IEMITSU, S. MAEDA, N. MESAKI, T. USHIDA, and T. AKIMOTO. Endurance Exercise Training Enhances Local Sex Steroidogenesis in Skeletal Muscle. Med. Sci. Sports Exerc., Vol. 43, No. 11, pp. 2072-2080, 2011. Purpose: Endurance training improves skeletal muscular function including energy metabolism and structure. Sex steroid hormones partly contribute to the exercise-induced muscular adaptations. Recently, we demonstrated that skeletal muscle contains steroidogenic converting enzymes to synthesize sex steroid hormones and an acute endurance exercise activates local steroidogenesis in skeletal muscle. However, whether chronic endurance training leads to enhanced steroidogenesis in skeletal muscle is unknown. Here, we examined changes in steroidogenic enzymes and sex steroid hormones in the skeletal muscle after chronic endurance exercise training. Methods: Eleven male rats were divided into two groups: sedentary (n = 6) and trained (n = 5). Endurance training was performed on a treadmill (30 m.min(-1), 30 min) for 5 d.wk(-1) for 12 wk. The posttraining harvesting was performed 48 h after the last exercise training. Results: The mRNA expressions of 3 beta-HSD, aromatase cytochrome P450, and 5 alpha-reductase in the skeletal muscle of trained rats were significantly higher than those of sedentary rats (P < 0.05). The protein expressions of aromatase cytochrome P450 and 5 alpha-reductase in the skeletal muscle of trained rats were also significantly higher than those of sedentary rats (P < 0.05). The muscular dihydrotestosterone (DHT) concentrations in the skeletal muscle of trained rats were significantly higher than those of sedentary rats (P < 0.01), but there was no change in dehydroepiandrosterone, total testosterone, free testosterone, and estradiol. Furthermore, muscle weight corrected for body weight of trained rats was moderately correlated with the level of muscular DHT concentration in trained rats (r = 0.41, P < 0.05). Conclusions: Endurance exercise training enhances the muscular DHT concentration through 5 alpha-reductase in the skeletal muscle of rats, suggesting that local bioactive androgen metabolism may participate in exercise training-induced skeletal muscular adaptation.