The influence of environmental factors on communities of arbuscular mycorrhizal fungi associated with Chenopodium ambrosioides revealed by MiSeq sequencing investigation.
The influence of environmental factors on communities of arbuscular mycorrhizal fungi associated with Chenopodium ambrosioides revealed by MiSeq sequencing investigation.
复制标题
MiSeq测序揭示环境因素对土藜相关丛枝菌根真菌群落的影响
DOI：
10.1038/srep45134
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发表时间：
2017-03-22
影响因子：
4.6
通讯作者：
Shen Z
中科院分区：
文献类型：
--
作者：
Xu X;Chen C;Zhang Z;Sun Z;Chen Y;Jiang J;Shen Z
丛枝菌根真菌（AMF）影响多种生态系统功能和过程，其组合因生态系统而异。然而，环境因素对AMF群落的影响在很大程度上仍然是未知的。本研究对不同自然土壤条件下的羊绒藜根际土壤和根际土壤AMF群落进行了研究。与根际土壤生境相比，根际土壤生境的otu数量和群落丰富度显著减少。根生境的大部分otu与同一采样点的土壤生境共享，表明根际土壤是AMF物种的一个库，其中一部分由植物吸收。根生境中AMF以肾小球科植物居多，表明植物对AMF的招募偏好。评估了环境因子对AMF群落组成和系统发育结构变化的相对贡献。结果表明，土壤性质是主要的解释因素，其次是地理和气候参数，它们单独解释了一小部分，而寄主植物的解释作用很少。总体而言，土壤和根系生境以及土壤性状，特别是pH、氮和微量元素（Zn和Cu）对AMF群落有显著影响。
Arbuscular mycorrhizal fungi (AMF) affect multiple ecosystem functions and processes, the assemblages of which vary across ecosystems. However, the influences of environmental factors on AMF communities which may shape these communities are still largely unknown. In this study, AMF communities from roots and rhizosphere soils of Chenopodium ambrosioides in different natural soils were investigated. The root habitat showed significantly smaller numbers of OTUs and lower community richness compared to the rhizosphere soil habitat. Most OTUs in the root habitat were shared by the soil habitat from the same sampling site, indicating that rhizosphere soils represent a pool of AMF species, a fraction of which is recruited by plants. Most of the AMF in root habitats were Glomeraceae, suggesting recruitment preferences of AMF by plants. The relative contributions of environmental factors to explain variations in AMF community composition and phylogenetic structure were assessed. The results revealed soil properties predominantly explained the variation, followed by geographic and climate parameters which explained a small fraction independently, while the host plant showed few explanations. Overall, our results indicated that soil and root habitats as well as soil characters, especially pH, nitrogen and micronutrients (Zn and Cu) affected AMF communities significantly.