Structural Characterization and Immunostimulatory Activity of Heteropolysaccharides from Fuzhuan Brick Tea
Structural Characterization and Immunostimulatory Activity of Heteropolysaccharides from Fuzhuan Brick Tea
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DOI：
10.1021/acs.jafc.0c06913
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发表时间：
2021-01-22
影响因子：
6.1
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
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作者：
Chen, Guijie;Bai, Yixun;Liu, Zhonghua
附砖茶是一种独特的黑茶，具有多种保健功能。本研究从FBT中分离纯化出一种多糖组分FBTPS-2-1，并对其结构和潜在的免疫刺激活性进行了研究。结果表明，FBTPS-2-1是一种典型的杂多糖，主要由Gal、Ara和Glc组成，摩尔比为46.59：22.13：13.57：8.20：6.02：2.12：1.38，相对分子质量为748 kDa。FBTPS-2-1的主干含有-4)-β-D-Galp-(1->4)-β-D-Galp-(1->4)-β-D-Galp-(1->4)-α-D-Galp-(1->4)-α-D-Glcp-(1->4)-α-D-Glcp-(1->4)-α-D-Glcp-(1->；4)-α-D-Glcp-(1->4)-β-D-Galp-(1->，->3)-β-D-Galp-(1-&>4)-β-D-Galp-(1-&>，-3，6)-β-D-Galp-(1-&>3)-β-D-Galp-(1-&>；和-&>3，6)-β-D-Galp-(1-&>；3，6)-β-D-Galp-(1->；FBTPS-2-1中的支链主要由α-L-阿拉夫-(1->；3，6)-β-D-Galp-(1->；，->5)-α-L-Araf-(1->3，6)-β-D-Galp-(1->，->6)-β-D-Galp41->；3，6)-β-D-Galp-(1-&>；，-α-L-Araf-(1-&>；3，S)-α-L-阿拉夫-(1->；，->3，5)-α-L-阿拉夫-(1->5)-α-L-阿拉夫-(1->，α-D-Galp-(1->3，5)-α-L-阿拉夫-(1->；和->5)-α-L-阿拉夫-(1->6)-β-D-Galp-(1-&>此外，FBTPS-2-1还能增加巨噬细胞的吞噬功能，促进NO和多种炎性细胞因子的分泌，包括TNF-α、IL-1β和IL-6，具有明显的免疫增强活性。因此，FBTPS-2-1可以作为一种潜在的功能食品，通过调节宿主的免疫反应来改善人体健康。
Fuzhuan brick tea (FBT), one of the unique dark teas, has various health-promoting functions. In the present study, one polysaccharide fraction, namely FBTPS-2-1, was extracted and purified from FBT, and its structure and potential immunostimulatory activity were investigated. The results showed that FBTPS-2-1,one of typical heteropolysaccharides, was mainly composed of Gal, Ara, and Glc with little molar content of Man, Rha, GalA, and GlcA in molar ratio of 46.59:22.13:13.57:8.20:6.02:2.12:1.38 and molecular weight of 748 kDa. The backbone of FBTPS-2-1 contained -> 4)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 4)-beta-D-Galp-(1 -> 4)-alpha-D-Glcp-(1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-alpha-D-Glcp- (1 ->, -> 4)-alpha-D-Glcp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3)-beta-D-Galp-(1 -> 4)-beta-D-Galp-(1 ->, -> 3,6)-beta-D-Galp- (1 -> 3)-beta-D-Galp- (1 -> and -> 3,6)-beta-D-Galp-(1 -> 3,6)-beta-D-Galp-(1 ->. The linkages of branches in FBTPS-2-1 were mainly composed of alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 5)-alpha-L-Araf-(1 -> 3,6)-beta-D-Galp-(1 ->, -> 6)-beta-D-Galp41 -> 3,6)-beta-D-Galp-(1 ->, alpha-L-Araf-(1 -> 3,S)-alpha-L-Araf-(1 ->, -> 3,5)-alpha-L-Araf- (1 -> 5)-alpha-L-Araf-(1 ->, alpha-D-Galp-(1->3,5)-alpha-L-Araf-(1 -> and -> 5)-alpha-L-Araf-(1 -> 6)-beta-D-Galp-(1 ->. Furthermore, FBTPS-2-1 could increase the phagocytosis of macrophages and promote the secretion of NO and a variety of inflammatory cytokines, including TNF-alpha, IL-1 beta, and IL-6, indicating noticeable immune enhancement activity. Thus, FBTPS-2-1 could serve as a potentially functional food to improve human health by modulating the host immunoreaction.