2-LTR CIRCULAR VIRAL-DNA AS A MARKER FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION IN-VIVO
2-LTR CIRCULAR VIRAL-DNA AS A MARKER FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION IN-VIVO
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DOI：
10.1006/viro.1994.1667
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发表时间：
1994-12-01
期刊：
影响因子：
3.7
通讯作者：
GRAZIANO, FM
中科院分区：
文献类型：
--
作者：
PAUZA, CD;TRIVEDI, P;GRAZIANO, FM
在人类免疫缺陷病毒1型感染中发现的未整合的病毒DNA包括线性和环状形式。我们针对含有两个拷贝的病毒长末端重复序列（2-LTR环）的环状形式，并开发了特异性检测方法来检测HIV感染患者外周血单核细胞中的这种分子。在体外HIV-1感染的外周血单个核细胞显示快速积累和快速衰减的2-LTR环状病毒DNA。2-LTR环状病毒DNA水平的检查提供了基于病毒DNA形式的传播感染的观点，所述病毒DNA形式在结构上不同并且在感染细胞中具有已知的短半衰期。在未接受抗病毒治疗的患者中，2-LTR环状病毒DNA和总病毒DNA水平与CD 4细胞计数显著相关。在接受齐多夫定（AZT）、去羟肌苷（ddA）或扎西他滨（ddC）的患者中未观察到类似的相关性。(C)1994年出版社出版。
The unintegrated viral DNA found in human immunodeficiency virus type 1 infection includes linear and circular forms. We targeted the circular form containing two copies of the viral long terminal repeat (2-LTR circle) and developed specific assays to detect this molecule in peripheral blood mononuclear cells from HIV-infected patients. In vitro HIV-1 infection of peripheral blood mononuclear cells showed rapid accumulation and rapid decay of 2-LTR circular viral DNA. Examination of 2-LTR circular viral DNA levels provides a view of spreading infection based on a viral DNA form that is structurally distinct and has a known, short half-life in infected cells. In patients not receiving antiviral therapy, the levers of 2-LTR circular viral DNA and total viral DNA were significantly correlated to CD4 cell counts. similar correlations were not observed in patients receiving zidovudine (AZT), didanosine (ddA), or zalcitabine (ddC). (C) 1994 Academic Press, Inc.