Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7
Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7
复制标题
传染性胃肠炎病毒 (TGEV) 感染会改变影响 TGEV 基因 7 转录的细胞 MicroRNA 种类的表达
DOI：
10.7150/ijbs.11585
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发表时间：
2015-01-01
影响因子：
9.2
通讯作者：
Tong, Dewen
中科院分区：
文献类型：
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作者：
Song, Xiangjun;Zhao, Xiaomin;Tong, Dewen
传染性胃肠炎病毒（TGEV）是冠状病毒科的成员。 TGEV 感染已成为严重胃肠炎的主要原因，并导致许多细胞过程的改变。同时，TGEV的致病机制仍不清楚。 microRNA (miRNA) 是一类新型的小非编码 RNA，参与病毒感染和细胞凋亡等多种生物过程的调节。越来越多的数据表明，miRNA参与了冠状病毒感染的过程，例如严重急性呼吸综合征冠状病毒（SARS-CoV）的复制。然而，miRNA 与 TGEV 感染之间的联系尚不清楚。在这项研究中，我们进行了 microRNA 微阵列分析并预测了改变的 miRNA 的靶标。结果显示TGEV感染引起miRNA谱的变化。然后我们选择miR-4331进行进一步分析，随后确定细胞分裂周期相关蛋白7（CDCA7）作为miR-4331的靶标。此外，miR-4331 显示出通过直接靶向 CDCA7 抑制 TGEV 基因 7（一种非结构基因）转录的能力。总之，TGEV感染引起的差异表达的miR-4331可以通过靶向细胞CDCA7来抑制TGEV基因7的转录。我们的主要发现是 TGEV 选择性地操纵一些细胞 miRNA 的表达来调节其亚基因组转录。
Transmissible gastroenteritis virus (TGEV) is a member of Coronaviridae family. TGEV infection has emerged as a major cause of severe gastroenteritis and leads to alterations of many cellular processes. Meanwhile, the pathogenic mechanism of TGEV is still unclear. microRNAs (miRNAs) are a novel class of small non-coding RNAs which are involved in the regulation of numerous biological processes such as viral infection and cell apoptosis. Accumulating data show that miRNAs are involved in the process of coronavirus infection such as replication of severe acute respiratory syndrome coronavirus (SARS-CoV). However, the link between miRNAs and TGEV infection is unknown. In this study, we performed microRNA microarray assay and predicted targets of altered miRNAs. The results showed TGEV infection caused the change of miRNAs profile. Then we selected miR-4331 for further analysis and subsequently identified cell division cycle-associated protein 7 (CDCA7) as the target of miR-4331. Moreover, miR-4331 showed the ability to inhibit transcription of TGEV gene 7 (a non-structure gene) via directly targeting CDCA7. In conclusion, differentially expressed miR-4331 that is caused by TGEV infection can suppress transcription of TGEV gene 7 via targeting cellular CDCA7. Our key finding is that TGEV selectively manipulates the expression of some cellular miRNAs to regulate its subgenomic transcription.