A role for the vesicle tethering protein, p115, in the post-mitotic stacking of reassembling Golgi cisternae in a cell-free system.
A role for the vesicle tethering protein, p115, in the post-mitotic stacking of reassembling Golgi cisternae in a cell-free system.
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囊泡绑扎蛋白P115的作用在无细胞系统中重新组装高尔基硫酸盐的有序后堆叠中。
DOI：
10.1083/jcb.146.1.57
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发表时间：
1999-07-12
期刊：
影响因子：
--
通讯作者：
Warren G
中科院分区：
文献类型：
--
作者：
Shorter J;Warren G
在末期，高尔基体池从异质的管泡团群中再生和堆积。一个重建这些事件的无细胞系统揭示了脑池再生需要可溶性因子和可溶性N-乙基马来酰亚胺(NEM)敏感的融合蛋白(NSF)附着蛋白受体(SNARES)之间的相互作用，这些受体通过两条由ATPase控制的交叉通路p97和NSF。高尔基重组堆积蛋白65(GRASP65)是NEM敏感的膜结合组分，是堆积过程所必需的。NSF介导的脑池再生需要一个囊泡拴系蛋白p115，我们现在证明它是通过它的两个高尔基受体GM130和giantin起作用的。P97介导的脑池再生是不依赖p115的，但我们现在证明了p115及其受体在堆积p97产生的脑池中的作用。时间分析表明，p115在可能位于GRASP65介导的堆积的上游的堆积中起着短暂的作用。这些结果表明，p115及其受体参与了单池的初始对齐和对接，这可能是堆叠形成的重要先决条件。
During telophase, Golgi cisternae are regenerated and stacked from a heterogeneous population of tubulovesicular clusters. A cell-free system that reconstructs these events has revealed that cisternal regrowth requires interplay between soluble factors and soluble N-ethylmaleimide (NEM)–sensitive fusion protein (NSF) attachment protein receptors (SNAREs) via two intersecting pathways controlled by the ATPases, p97 and NSF. Golgi reassembly stacking protein 65 (GRASP65), an NEM-sensitive membrane-bound component, is required for the stacking process. NSF-mediated cisternal regrowth requires a vesicle tethering protein, p115, which we now show operates through its two Golgi receptors, GM130 and giantin. p97-mediated cisternal regrowth is p115-independent, but we now demonstrate a role for p115, in conjunction with its receptors, in stacking p97 generated cisternae. Temporal analysis suggests that p115 plays a transient role in stacking that may be upstream of GRASP65-mediated stacking. These results implicate p115 and its receptors in the initial alignment and docking of single cisternae that may be an important prerequisite for stack formation.