Regulation of plasma glycero-lysophospholipid levels by lipoprotein metabolism.
Regulation of plasma glycero-lysophospholipid levels by lipoprotein metabolism.
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DOI：
10.1042/bcj20190498
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发表时间：
2019-11
期刊：
影响因子：
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通讯作者：
M. Kurano;K. Kano;M. Hara;K. Tsukamoto;J. Aoki;Y. Yatomi
中科院分区：
文献类型：
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作者：
M. Kurano;K. Kano;M. Hara;K. Tsukamoto;J. Aoki;Y. Yatomi
甘油-溶血磷脂，如溶血磷脂酸和溶血磷脂酰丝氨酸，正引起人们的关注，因为特异性受体已被鉴定。这些化合物中的大多数已被提议与白蛋白结合，而它们与脂蛋白的关联尚未完全阐明。因此，在这项研究中，我们的目的是调查的内容甘油溶血磷脂（溶血磷脂酸，溶血磷脂酰胆碱，溶血磷脂酰乙醇胺，溶血磷脂酰甘油，溶血磷脂酰肌醇，溶血磷脂酰丝氨酸）的脂蛋白和脂蛋白代谢的调节。我们观察到，除溶血磷脂酰丝氨酸外，适量的甘油-溶血磷脂分布在LDL和HDL组分上，并且当它们共孵育时，在脂蛋白组分中观察到与白蛋白结合的甘油-溶血磷脂。胆固醇酯转移蛋白（CETP）的过度表达降低了小鼠血浆溶血磷脂酰胆碱、溶血磷脂酰乙醇胺、溶血磷脂酰甘油和溶血磷脂酰肌醇的水平，并增加了它们在载脂蛋白B中的含量，而降低了它们在HDL和脂蛋白耗尽组分中的含量。LDL受体的过度表达降低了溶血磷脂酰胆碱、溶血磷脂酰乙醇胺、溶血磷脂酰甘油和溶血磷脂酰肌醇的血浆水平，并降低了这些化合物在LDL、HDL和脂蛋白耗尽组分中的含量，而LDL受体的敲低则增加了它们。这些结果表明甘油溶血磷脂在脂蛋白的多效性作用中的潜在重要性以及脂蛋白代谢在甘油溶血磷脂调节中的重要性。
Glycero-lysophospholipids, such as lysophosphatidic acids and lysophosphatidylserine, are gathering attention, since specific receptors have been identified. Most of these compounds have been proposed to be bound to albumin, while their associations with lipoproteins have not been fully elucidated. Therefore, in this study, we aimed to investigate the contents of glycero-lysophospholipids (lysophosphatidic acids, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, and lysophosphatidylserine) on lipoproteins and the modulation of their metabolism by lipoprotein metabolism. We observed that moderate amounts of glycero-lysophospholipids, with the exception of lysophosphatidylserine, were distributed on the LDL and HDL fractions, and glycero-lysophospholipids that had bound to albumin were observed in lipoprotein fractions when they were co-incubated. The overexpression of cholesteryl ester transfer protein (CETP) decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and it increased their contents in apoB-containing lipoproteins, while it decreased their contents in HDL and lipoprotein-depleted fractions in mice. The overexpression of the LDL receptor decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and decreased the contents of these compounds in the LDL, HDL, and lipoprotein-depleted fractions, while the knockdown of the LDL receptor increased them. These results suggest the potential importance of glycero-lysophospholipids in the pleiotropic effects of lipoproteins as well as the importance of lipoprotein metabolism in the regulation of glycero-lysophospholipids.